Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2010, Vol. 06 ›› Issue (02): 115 -118. doi: 10.3877/cma.j.issn.1673-5250.2010.02.014

Original Article

Expression of Focal Adhesion Kinase in Epithelial Ovarian Cancer and Its Clinical Significance

Xing-li XU, Qing-lan JIA, Xiao-hua YAN, Xiang-ning ZHANG   

  1. Department of Obstetrics and Gynecology, Tengzhou Central People's Hospital, Tengzhou 277500, Shandong Province, China
  • Published:2010-04-01
Objective

To investigate the relationship between the expression of focal adhesion kinase (FAK) in human epithelial ovarian cancer (EOC) and clinicopathological features.

Methods

From June 2004 to December 2007, western-blot was used to detect the expression of focal adhesion kinase in 56 epithelial ovarian cancer with different clinical stages and different degrees of differentiation (research group). Research group was furthermore divided into following sub-groups: Lymph node metastasis sub-group (n=37), and non-lymph node metastasis sub-group (n=19); <50-year-old group (n=27) and ≥50-year-old group (n=29); T1, T2 sub-group (n=15) and T3, T4 sub-group (n=41) according to the clinical staging of malignant tumors made by International Federation of Gynecology and Obstetrics (FIGO) in 2000; grade Ⅰ sub-group (n=16), grade Ⅱ sub-group (n=25), and grade Ⅲ sub-group (n=15) according to the principle of pathological classification of malignant tumors; serous sub-group (n=30), mucinous sub-group (n=16), and endometrioid carcinoma sub-group (n=10) by different histological types. Another 22 cases with ovarian benign tumors were included to control group, including 14 cases with serous papillary cystadenoma and 8 cases with mucinous papillary cystadenoma. Informed consent was obtained from all participants. The positive expression rate of focal adhesion kinase between research group and control group was compared. The relationship between expression levels of focal adhesion kinase and clinicopathological characteristics were analyzed by χ2 test and Fisher exact test.

Results

The positive rate of focal adhesion kinase in research group was 76.9%(43/56) and in control group was 9.1%(2/22) (P<0.05). Significant difference was found in the positive expression rate of focal adhension kinase with different clinical staging (P<0.05). There was significant difference of different clinical stages between node metastasis sub-group and non-metastasis sub-group (P<0.05). There were no significant differences in the expression of focal adhesion kinase with different age of patients, pathologic grades and histologic types of tumors (P>0.05).

Conclusion

The expression level of focal adhesion kinase in ovarian cancer is higher than that of benign tumors. The positive expression rate of focal adhesion kinase has a close relationship with clinical staging and lymph node metastasis of epithelial ovarian cancer, but it has no relationship with age, pathological grades and histological types of tumors.

图1 黏着斑激酶在研究组和对照组中的表达(1:卵巢良性肿瘤;2:上皮性卵巢癌;3:卵巢良性肿瘤β-actin;4:上皮性卵巢癌β-actin)
图2 研究组及对照组中黏着斑激酶的表达及研究组中黏着斑激酶表达与临床病理特征间的关系(图2-A N:良性卵巢组织;T1~T4:T1~T4期上皮性卵巢癌。图2-B N:良性卵巢组织;T1~T3:卵巢上皮癌病理学分级Ⅰ~Ⅲ级)
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