Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2009, Vol. 05 ›› Issue (05): 466 -469. doi: 10.3877/cma.j.issn.1673-5250.2009.05.102

Original Article

Dynamic Changes of T Lymphocyte Subset and B Cells in Children With Infectious Mononucleosis Caused by Epstein-Barr Virus

Shu-hong RENG, Qiang WANG, Jun FANG, Jing LI   

  1. Department of Pediatrics, Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Published:2009-10-01
  • Supported by:
    * Project No. 303005002153009, supported by Sichuan Provincial Health Department Research Project
Objective

To study the dynamic changes of T lymphocyte subset and B cell in children with infectious mononucleosis(IM) caused by Epstein-Barr virus (EBV).

Methods

From March 2006 to March 2008, 30 children patients with infectious mononucleosis (study group) and 25 sex- and age-matched health children (control group) without allergy history were enrolled in this study. Informed consent was obtained from all participates. There had no significant difference of age and gender between study group and control group (P>0.05). The samples of peripheral blood (2 mL) in study group were collected in acute phase, convalescent phase (at the first month, third month, and sixth month) and the same to control group. All samples were analyzed the expression of CD3+ , CD4+ , CD8+ , CD19+ and CD19+ CD23+ on peripheral blood lymphocytes by flow cytometry.

Results

① Compared with control group, the expression of CD4+ [(14.84±5.03)%] and CD4+ /CD8+ [(0.25±0.13)%], CD19+ [(3.89±1.32)%] and CD19+ CD23+ [(0.24±0.13)%] were markedly decreased in the acute stage of study group. One way analysis of variance among five groups had significant difference (P<0.001). The expression of CD3+ [(82.55±5.49)%] and CD8+ [(66.17±8.10)%] were obviously increased in the acute stage of study group compared with control group (P<0.001). ②As time went by and the infection was controlled, CD4+ , CD4+ /CD8+ , CD19+ , and CD19+ CD23+ of study group raised increasingly than those of control group, CD3+ , CD8+ reduced decreasingly in study group. ③There were no significant difference on the expression of CD3+ , CD4+ , CD8+ , CD4+ /CD8+ and CD19+ CD23+ between study group at the sixth month and control group (P>0.05), but the expression of CD19+ of study group at the sixth month was lower than that of control group(P<0.001).

Conclusion

The expression of CD4+ , CD4+ /CD8+ , CD19+ and CD19+ CD23+ were markedly decreased in the acute stage. The expression of CD3+ , CD8+ were obviously increased in the acute stage. The expression of CD3+ , CD4+ , CD8+ , CD4+ /CD8+ and CD19+ CD23+ returned to normal at the sixth month, but the expression of CD19+ did not return at the sixth month. Children patients with infectious mononucleosis have secondary humoral immunosuppression, which continued for long time after the recovery. This study shows an experimental evidence that treated the disease with immunoglobulin.

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