Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2009, Vol. 05 ›› Issue (01): 39 -42. doi: 10.3877/cma.j.issn.1673-5250.2009.01.111

Original Article

Interleukin-1β Leads to Lungs Injury in Rats With Renal Ischemia Reperfusion Injury.

Ning-kang BI, Yi-nan YUE, Hong-yu JIANG, Li ZHANG   

  1. Department of Internal Medicine, Second Hospital of Jining, Jining 272049, China
  • Published:2009-02-01
Objective

To investigate the expression of serum interleukin-1β(IL-1β) and the apoptosis of lungs in rats with renal ischemia reperfusion(IR).

Methods

Forty-eight Sprague-Dawley rats were divided into 6 groups randomly, including IR groups (IR 2 h group, IR 6 h group, IR 12 h group, IR 24 h group, IR 72 h group) and the sham control group. The animal models of IR groups were set up by the operation of 45 minutes bilateral renal pedicle clamping and later reperfusion after 2 h, 6 h, 12 h, 24 h, 72 h on Sprague-Dawley rats. Samples for serum and the left lung of each animal were taken. The serum sample was for the analysis of the expression of interleukin-1β, scr by the method of ELISA, and the lung sample for apoptosis and histological examination.

Results

The expression levels of serum interleukin-1β, scr and apoptosis positive cells of lungs were gradually increased from the sham control group to IR 12 h group. The number of cells in lung underwent apoptosis was obviously increased in IR 12 h group than that in the sham control group and other IR groups. The pathological lesions of lungs were most severe at IR 12 h group.

Conclusion

The expression levels of serum interleukin-1β, scr and apoptosis positive cells of lungs in rats with renal ischemia reperfusion are increased.

图1 假手术对照组HE染色(40×10)
图2 IR12 h组HE染色(40×10)
图3 假手术对照组细胞凋亡(40×10)
图4 IR12 h组细胞凋亡(40×10)
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