Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2009, Vol. 05 ›› Issue (01): 23 -28. doi: 10.3877/cma.j.issn.1673-5250.2009.01.108

Original Article

Correlation Study Between the Myocardial Anoxia Ischemia Reperfusion Injury and Myocardium Remodeling in Asphyxiated Newborn Rats.

Shu–zhen XU, Cai–xia WANG, Wei ZHAO, Jian–fang CHU   

  1. Department of Pediatrics, Qingdao Municipal Hospital, Qingdao 266011, China
  • Published:2009-02-01
Objective

To observe the expression of matrix metalloproteinases (MMPs) and transforming growth factor-β1 (TGF-β1) and their effectiveness on myocardial repairing and remodeling after anoxia ischemia reperfusion injury in rats.

Methods

The anoxia ischemia reperfusion injury animal model was set up in this study. Sixty Wistar rats were randomly divided into 4 groups: the group A, B, C (at the first day, the seventh day and the fourteenth day after the myocardial anoxia ischemia reperfusion injury) and the control group(without any asphyxia treatment)(15 rats for each group). The blood serum cardiac troponin Ⅰ (cTnⅠ) was measured by the rapid quantifying. Masson staining was used to study the collagen volume fraction (CVF), and myocardial histopathological integration was tested by the HE staining. The myocardial matrix metalloproteinase-3, -9 activities was measured by the immunohistochemical assay, and the transforming growth factor-β1 expression of myocardium were measured by the immunohistochemistry assay and semiquantitative analysis.

Results

Serum cardiac troponin Ⅰ provisionality raised, the level of the group A were obviously higher than those of the control group (P<0.01), but the level of the group B and C declined (P>0.05); matrix metalloproteinase-3 activity in the group A, B and C were significantly higher than those of the control group, reaching a peak on the group B (P<0.05). The metalloproteinase-9 activity in the group A, B and C were significantly higher than those of the control group (P<0.05). Collegen volume fractions were gradually increased after asphyxia(P>0.05, P<0.05, P<0.05, respectively) and the group C reached the highest level. The expression of transforming growth factor-β1 enhanced by the change of the time(P>0.05, P<0.01, P<0.01, respectively). Collegen volume fractions were positively correlated with transforming growth factor-β1 (r=0.574, P<0.01). Matrix metalloproteinase-3, -9 activities positively correlated with collegen volume fractions (r=0.482, 0.679; P<0.05).

Conclusion

There has anoxia ischemia reperfusion injury in rats myocardium. After the asphyxia, the matrix metalloproteinase-3, -9 activities are activited and the expression of transforming growth factor-β1 are enhanced, myocardial collagens increase. Matrix metalloproteinase-3, -9, transforming growth factor-β1 may relate with the myocardial remodeling after the anoxia ischemia reperfusion injury.

图1 MMP-3免疫组化阳性表达(S-P×100)
图2 MMP-9免疫组化阳性表达(S-P×200)
图3 HE染色心肌细胞肥大
图4 HE染色胶原增生
图5 免疫组化TGF-β1阳性
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