Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2009, Vol. 05 ›› Issue (01): 13 -17. doi: 10.3877/cma.j.issn.1673-5250.2009.01.106

Original Article

Expression of the Neuropeptide Y and Leu–Enkephalin in the Brain of Pentylenetrazol–Induced Epileptic Rat Models.

Lang CHEN, Qiao–bin CHEN, Zhang–yu ZOU, Li–fang LIN, Xiao–li SHEN, Xin CHEN, Xiao–lin JI   

  1. Department of Pediatrics, Fujian Provincial Hospital, Fuzhou 350001, China
  • Published:2009-02-01
  • Supported by:
    * Project No.Z0516068, supported by the Fujian Provincial Natural Science Funding
Objective

To evaluate the significant changes of the neuropeptide Y(NPY) and leu-enkephalin(L-Enk) in the brain of pentylenetetrazol(PTZ)-induced rats in order to understand the relationship among the neuropeptide Y, leu-enkephalin and epilepsy.

Methods

Fifty male adult SD rats were randomly divided into the experimental group (group A, n=40, intraperitoneal pentylenetetrazol injection 50 mg/kg) and the control group (group B , n=10, intraperitoneal tales doses normal saline injection). According to the modification of seizure activity, the group A was divided into non-seizure group (group A1, n=7) which was decapitated immediately and the seizure group(group A2~A5, n=33). Rats of the seizure group were decapitated immediately (group A2, n=10), at the sixth hour(group A3, n=11), at the 24th hour(group A4, n=10) and at the 72th hour(group A5, n=2) after the seizure. Radioimmunoassay (RIA) and immunocytochemical techniques were used to evaluate the changes of the neuropeptide Y and leu-enkephalin in mesencephalon, hippocampus, striatum, and frontal cortex.

Results

The level of the neuropeptide Y increased significantly in mesencephalon(P<0.05), frontal cortex(P<0.05), and striatum (P<0.01) of the group A in contrast to those of the group B, and no difference was found in hippocampus. The level of the neuropeptide Y in brains of the seizure group(group A2~A5, n=33) was significant higher than that of the group B. The level of the neuropeptide Y returned to the value of the non-seizure group at the 24th hour after the seizure. Both groups had an elevated level of the leu-enkephalin in mesencephalon, striatum, hippocampus and frontal cortex. No difference was found between the non-convulsive group and the convulsive group in the four brain areas above, but the leu-enkephalin content of hypothalamus in the convulsive group was higher than that in the non-convulsive group. After the convulsion, the level of the leu-enkephalin decreased.

Conclusion

The increased level of the neuropeptide Y and leu-enkephalin may play an important role during the epilepsy.

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