Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2008, Vol. 04 ›› Issue (02): 91 -96. doi: 10.3877/cma.j.issn.1673-5250.2008.02.106

Original Article

The Change of Notch Signal Rats in Lung Tissues of Pulmonary Hypertension

Li-na QIAO, Kun SHI, Xian-min WANG, Bin LIU, Yi-min HUA, Han-min LIU, Tong-fu ZHOU   

  1. The Second West China Hospital of Sichuan University, Chengdu 610041, China
  • Published:2008-04-01
Objective

To explore the change of Notch signal in rats' lung tissues of pulmonary hypertension(PH).

Methods

The PH models of rats were established by pneumonectomy plus monocrotaline injection, which was compared indexes about pulmonary vascular remodeling with the group of normal rats. Their lung tissues were detected by immunohistochemistry technique and real-time fluorescent quantitation RT-PCR for Notch 1, 2, 3 and 4 receptor.

Results

Those indexes of the model group were higher than the normal group, including mean pulmonary arterial pressure(mPAP), fulton index [RV/(LV+ S)], percentage of small pulmonary arteries media thickness(WT, %), muscularizition of non-musclirised pulmonary arterioles and extent of cellular proliferation in pulmonary arterioles (P<0.05). And neointima formation was observed in model group. By immunohistochemistry staining, Notich 1, Notich 3, Notich 4 receptor were positive in pulmonary arteries of the model group and the normal group. Notch 1 and 3 receptor were positive in smooth muscle cells of pulmonary arteries, and Notch 1 and 4 receptor were positive in endothelial cells of pulmonary arteries, while Notch 2 receptor was negative in pulmonary arteries. Notch 1, 2, 3 and 4 receptor mRNA levels in lung tissues of model group were higher than the normal group.

Conclusion

Notch 1, 3 and 4 receptor were expressed in pulmonary artery, which up-regulation seemed related to the development of pulmonary vascular remodeling during PH, and possibly involved in pulmonary vascular remodeling.

图1 大鼠肺组织Notch 1受体免疫组化染色,示肺小动脉(400×)
图2 大鼠肺组织Notch 2受体免疫组化染色,示肺小动脉(400×)
图3 大鼠肺组织Notch 3受体免疫组化染色,示肺小动脉(400×)
图4 大鼠肺组织Notch 4受体免疫组化染色,示肺小动脉(400×)
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