Pooling urine plasmid gap-LCR-ELISA to detect Chlamydia trachomatis infection in pregnant women

doi:10.3877/cma.j.issn.1673-5250.2007.04.104

Objective

To construct an effective pooling urine plasmid gap-LCR-ELISA method for large-scale epidemic screening of perinatal Chlamydia trachomatis (CT) infection.

Methods

The most optimal pooling size (4×, 6×, 8×, 10×) was chosen by comparing the sensitivity, specificity and cost-effectiveness with single urine detection.

Results

Pooling urine by 4×, 6×, 8×, 10× size to gap-LCR-ELISA resulted in a satisfying specificity up to 100%, but the sensitivity declined with the increase of pooling volume (95%, 85%, 70% and 65%, respectively). And a significant cost reduction of more than40% was obtained in all the groups.

Conclusion

4× size pooling urine strategy could achieve the same detecting capability as that of individual specimen examined by plasmid gap-LCR-ELISA, which is quite valuable in large scale epidemic screening for CT infection.

Key words: Chlamydia trachomatis, plasmid, gap-LCR-ELISA, urine

图1　混合尿液gap-LCR-ELI SA法比较

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