Effects of the neonatal handling and the enriched environment on nerve growth factor and its receptor Trk A in the brain of neonatal rats

doi:10.3877/cma.j.issn.1673-5250.2007.03.108

Objective

To explore the effects of the neonatal handling and the enriched environment on nerve growth factor (NGF) and its tyrosine kinase receptor A (Trk A) in the forehead cortex and hippocampus of neonatal rats.

Methods

60 neonatal rats were divided into the early intervention group and the control group randomly. The rats in early intervention group received the neonatal handling for14 d and then were kept in an enriched environment for another 14 d. NGF levels were determined by an enzyme-linked immunosorbent assay (ELISA) method and positive the cells expressed Trk A were stained by immunohistochemistry.

Results

In the forehead cortex and hippocampus, NGF levels were(2343. 58±133. 15) pg/g and(1547. 42 ± 65. 32) pg/g in the intervention group, which were much higher than those in the control group [(2174. 70±165. 21) pg/g and(1475. 23 v 76. 72)pg/g] (P<0. 05). The numbers of neurons labelled with Trk A antibody in the forehead cortex and hippocampus of the intervention group were (80. 56 ± 20. 12)cells/HF and(66. 42 ± 21. 83)cells/HF, which were more than those in the control group [(63. 50±18. 23)/HF and(50. 45 ± 17. 28)/HF](P<0. 05).

Conclusion

The neonatal handling and the enriched environment may improve brain development by increasing the levels of endogenetic NGF levels and Trk A expressed in the brain.

Key words: neonatal handling, enriched environment, nerve growth factor (NGF), tyrosine kinase receptor A(Trk A), rats

图1　干预组与对照组海马CA₃区Trk A受体免疫反应神经元

	1　鲍秀兰主编.新生儿行为能力和0〜3岁早期教育.第1版.北京：中国少年儿童出版社，1995，212-219,338-351.
	2　Young D，Lawlor PA，Leone P，et al. Environment enriched inhibits spontaneous apoptosis，prevent seizures and isneuroprotetive. Nat Med,1999,5：448-453.
	3　Philips MF，Mattiasson G, Wieloch T，et al. Neuroprotective and behavioral efficacy of nerve growth factor-transfected hippocampal progenitor cell transplants after experimental traumatic brain injury. Neurosurg，2001，94：765-774.
	4　Takatori M，Kuroda Y，Hirose M. Local anesthetics suppress nerve growth factor-mediated neurite outgrowth by inhibition of tyrosine kinase activity of TrkA. Anesth Analg，2006，102: 462-467.
	5　Meancy MJ, Aitken DH, Berkel CV, et al. Effect of Neonatal handling on age-related impairments associated with the hippocampus. Science,1988，239: 766-768.
	6　Pham TM, Soderstrom S, Henriksson BG, et al. Effects of neonatal stimulation on later cognitive function and hippocampal nerve growth factor. Behav Brain Res, 1997，8:113-120.
	7　Soderstrom S，Hallbook F，Ibanez CF, et al. Recombinant human beta-nerve growth factor ( NGF)：Biological activity and properties in an enzyme immunoassay. Neurosci Res, 1990，27:665-677.
	8　Dekosky ST, Ikonomovic MD, Styren SD. Upregulation of choline acetyltransferase activity in hippocampus and frontal cortex of elderly subjects with mild cognitive impairment. Ann Neurol,2002,51:145-155.
	9　Fagan AM，Garber M，Barbacid M，et al. A role for TrkA during maturation of striatal and basal forebrain cholinergic neurons in vivo. J Neurosci, 1997,17:7644-7654.
	10　Sofroniew MV, Howe CL，Molliam WC. Nerve growth factor signaling, neuroprotection, and neural repair. Annu Rev Neurosci,2001,24：1217-1281.
	11　Thorne RG，Frey WH. Delivery of neurotrophic factors to the central nervous system：Pharmacokinetic considerations. Clin Pharmacokinet，2001,40(12)：907-946.
	12　Wagner AD, Schacter DL, Rotte M, et al. Building memories：Remembering and forgetting of verbal experiences as predicted by brain activity. Science, 1998,281:1188-1191.

[1]	Hongxin Shi, Zhifang Tang, Minzheng Guo, Luqiao Pu, Baochuang Qi, Junxiao Ren, Chuan Li. Exploration of methodology and role of sodium iodoacetate in modeling of rat osteoarthritis[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2023, 17(04): 534-539.
[2]	Maoxia Liu, Yanbing Zhang, Zhengda Li, Jun Jin, Xinjing Yang. Protective effect of esmolol on sepsis-induced acute myocardial injury in rats[J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2022, 15(06): 448-453.
[3]	Yan Zhang, Jun Wang, Yongxing Yan, Jing Gu, Yingnan Wei, Yonghui Shen. Protective effect and mechanism of 3-acetyl-11-keto-β-boswellic acid on cerebral ischemia-reperfusion injury in rats[J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2021, 14(06): 466-471.
[4]	Xiaoyan Zhang, Dongqiong Xiao, Hu Gao, Lin Chen, Fajuan Tang, Xihong Li. The protective effect and mechanism of overexpression of transcription factor 12 on the cerebral cortex of rats with sepsis-associated encephalopathy[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 540-549.
[5]	Xiya Jin, Xiaosong Huang, Cheng Tan, Qin Jiang, Fang Hou, Yaoyue Li, Bing Xu, Honghui Jia, Wenying Liu. Antenatal treatment of tacrolimus on pulmonary vascular remodeling in rat with experimental pathological model of congenital diaphragmatic hernia[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(04): 428-436.
[6]	Meidi Zhu, Shuming Wang, Sijia Guo, Weibin Jing, Mingming Ma, Rui Liu. Protective effect of sodium valproate on lung function in rats with 50% total body surface area Ⅲ degree scalded injury[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(01): 47-52.
[7]	Weixia Cai, Tao Cao, Ming Zhao, Dan Xiao, Yanhui Jia, Jing Wang, Yue Zhang, Kejia Wang, Juntao Han, Dahai Hu. Effects of Notch signaling pathway on the intercellular adhesion molecule-1 of pulmonary vascular endothelial cell induced by serum in burned rats[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(04): 292-299.
[8]	Qiang Chen, Shengjun Cao, Te Ba, Cuijuan Zhao. Effect and related mechanism of faecal microbiota transplantation on intestinal barrier function in severely burned rats[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(04): 322-329.
[9]	Rui Liu, Sijia Guo, Weibin Jing, Mingming Ma, Shuming Wang. Protective effect of histone deacetylase inhibitor valproate on cerebral injury in fatally scalded rats[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(01): 32-39.
[10]	Ming Li, Tiantian Liu, Hanting Zhu, Yong Fang, Tao Ni. Effects of LED red light on wound healing in diabetic rats[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(01): 18-24.
[11]	Qiang Wei, Mingxiang Zhang, Qiangpu Chen, Baofang Sun. Effect of modified xiaochengqi decoction on the gastrointestinal motility of rats with obstructive jaundice[J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(04): 267-270.
[12]	Wancheng Shi, Wende Yang, Qianhui Liu, Xu'na Huang, Xiaofeng Yang, Shangbin Yang, Jiafeng Fang. Establishment of hydrogen peroxide-induced rat major pelvic ganglion injury model in vitro[J]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(06): 401-405.
[13]	Qiang Lu, Lifei Yang, Kang Liu, Jiawei Yu, Lu Ren, Xufeng Zhang, Yi Lyu. Experimental study of liver transplantation without graft irrigation or perfusion in rat models[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(01): 103-107.
[14]	Yuzhou Long, Hua Liu, Yunqian Zhang, Xingtong Li, Yunhu Fan, Zhengliang Shang, Zhenyu Song, Lihua Luo. Study on edaravone pretreatment prolonging thrombolytic time window by acute ischemic stroke and mechanism of ROS/TXNIP/NLRP3 pathway in rat[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2023, 13(02): 65-74.
[15]	Jinying Zhang, Yanjun Zhang. Effect of Fuzheng Xiaowei Decoction on gastrointestinal motility and gastric mucosa repair in chronic atrophic gastritis rats induced by N-Methyl-N'-nitro-N-nitrosoguanidine[J]. Chinese Journal of Gastroesophageal Reflux Disease(Electronic Edition), 2023, 10(01): 11-17.

Viewed

Full text

Abstract

Please choose a citation manager

Content to export