Chinese Medical E-ournals Database

Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition) ›› 2007, Vol. 03 ›› Issue (02): 91 -f11. doi: 10.3877/cma.j.issn.1673-5250.2007.02.110

Original Article

Effects of early intervention on electroencephalogram and surviving neurons of infant rats with brain damage

Lin LIU, Yan-hui CHEN, Da-guang CHEN   

  1. Pediatric department, the Union Hospital affiliated with Fujian Medical University, Fuzhou, 350001, China
  • Published:2007-04-01
Objective

To explore whether early intervention can affect electroencephalogram (EEG) and surviving neurons of infant rats with brain damage or not.

Methods

A rat model with HIBD in uterus was set up. Animals were divided into four groups (the intervention group treated by HIBD, HIBD-IT; the non-intervention group treated by HIBD, HIBD-NIT; the control with intervention group, Control-IT, the control with non-intervention group, Control-NIT) at random. After 24 h of the operation, the rats of the intervention groups received stimuli of handling, hearing and sight for 14 days, and then they were kept in an enriched environment for 14 day. The rats' functional outcome was observed the nerve electricity physiology changes in the central nervous system (CNS) by electroencephalogram. The tapetum histopathology variety was observed in the microscope by the Nissl's staining.

Results

In brain waves of HIBD-IT rats, we observed not only less background rhythm disordered, but also polymorphic slow waves with high amplitude occurred less frequently than that of HIBD-NIT rats, Surviving neurons of the HIBD-IT rats were more than the HIBD-NIT rats by Nissl's staining.

Conclusion

The comparision of EEG and surviving neurons indicated that early intervention might improve the development of infant rats with brain damage.

图1 正常非干预组第29 d大鼠的EEG
图2 正常干预组第29 d大鼠的EEG
图3 HIBD干预组第29 d大鼠的EEG
图4 HIBD非干预组第29 d大鼠的EEG
图5 HIBD非干预组尼氏染色(× 400)
图6 HIBD干预组尼氏染色(× 400)
图7 正常干预组尼氏染色(×400)
图8 正常非干预组尼氏染色(× 400)
1 Tan S,Parks DA. Preserving brain function during neonatal asphyxia. ClinPerinatol,1999,26(3) : 733-747.
2 陈燕惠,陈达光,陈珊等.婴儿早期教育实验研究.中国优生优育杂志,1998,9(2):72-74.
3 Bjelke B,Andersson K, Ogren SO,et al. Asphyctic lesion:Proliferation of tyrosine hydroxylase-immunoreactive nerve cell bodies in the rat substaintia nigra and functional changes in dopamine neurotransmission. Brain Res, 1991,543(1):1-9.
4 Bona E,Johansson BB, Hagberg H. Sensorimotor function andneuropathology five or six weeks after hypoxia-ischemia in seven-day-old rats. Pedistric Res,1997,42(5):678-668.
5 Ickes BR,Pham TM,Sanders LA,et al. Long-term environment leads to regional increase in neurotrophin levels in rat brain. Exp Neurol, 2000,164(1):45-52.
6 Pham TM,Ickes BR,Albeck D,et al. Changes in brain nerve growth factor levels and nerve growth factor receptors in rats exposed to environmental enrichment for one year. Neuroscience,1999,94(1):279-228.
7 Femandaz V, Adaro L, Sanhuezs-Tsutsumi M,et al. Early-life polysensorial stimulation and nutrition:Topographic levels of susceptibility in the rats visual cortex. Biol Neonate, 1997,71(4):265-276.
8 Golanov Ev,Reis DJ. Neurons of nucleus of the solitary tract synchronize the EEG and elevate cerebral blood flow via a novel medullary area. Brain Res, 2001,892(1):1-12.
9 CFA卡特著(英),孔庆雷译.组织病理学与组织化学技术手册.北京:中国科技出版社,1982.
10 王平宇主编.大白鼠中枢神经系统解剖学基础.北京:人民卫生出版社,1986,465.
11 John RH著.马仁飞译.临床实用脑电图学.北京:人民卫生出版社,1997,172-176.
12 D'Arceuil H, Rhine W,de Crespigng A, et al. 99mTc annexin Vimaging of neonatal hypoxic brain injury. Stroke, 2000,31(11):2692-2700.
13 陈敏,胡君,陈燕惠等.早期干预促进大鼠神经生长因子表达与脑功能的关系.中国误诊学杂志,2003,3(3):321-323.
14 刘玲,陈达光,陈燕惠.早期干预对宫内缺氧缺血大鼠脑功能及神经生长相关蛋白表达的影响.中华物理医学与康复杂志,2005,27(1):20-23.
15 陈敏榕,陈燕惠,陈达光.早期干预对缺氧缺血脑损伤大鼠脑功能及神经元凋亡的影响.福建医科大学学报,2004,38(3):321-323.
[1] Liping Yao, Yan Li, Qiufen Wei, Danhua Meng, Kaiyan Shen, Hongjuan Bi, Lianfang Jing, Wei Tan. Effects of early neurodevelopment plan for premature infants on neurodevelopment: a prospective study[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2020, 16(05): 590-596.
[2] Haiyan Huang, Anfeng Lu, Guosheng Huang, Xuelan Bi. Influences of early intervention with new follow-up pattern on the neurodevelopment of preterm infants[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2018, 14(03): 331-336.
[3] Bingling Zhao, Lihong Wei, Jihong Wei. Psychological survey and psychological interventions in infertility patients with assisted reproductive technology[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2016, 12(02): 245-248.
[4] Dan HE. Influence of Ultra-Early Intervention on the Physical and Intellectual Development of Premature Infants[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2012, 08(01): 54-56.
[5] Kequn Huang, Lin Liu, Wei Cui, Xiang Wu. Research progress in microRNA regulating cognitive function[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2020, 10(01): 53-56.
[6] Xinxian Liu, Yaqi Wang, Bin Zhou, Yanyan Guo. The significance of rapamycin in early intervention of erosive esophageal stenosis in rabbits[J]. Chinese Journal of Interventional Radiology(Electronic Edition), 2023, 11(04): 324-329.
[7] Yue Zhang, Haozhi Qin, Ting Wang, Yuan Niu, Yao Ge, Lixue Qiao, Ailin Du. Effects of acanthopanax senticosus injection on the learning and memory ability and the expression of f-actin in hippocampus of brain hypoxia rats[J]. Chinese Journal of Diagnostics(Electronic Edition), 2018, 06(04): 272-276.
Viewed
Full text


Abstract