The Study of hypoxia-inducible transcription factor-1α in the placenta with intrahepatic cholestasis of pregnancy

doi:10.3877/cma.j.issn.1673-5250.2006.05.104

Objectives

We investigated the expression of the hypoxia-inducible transcription factor(HIF)in placenta of pregnant women with intrahepatic cholestasis of pregnancy(ICP).

Methods

HIF-1α and HIF-2α Proteins from villous explant culture under hypoxia condition were probed by immuno-histochemistry methods.

Results

① HIF-1α protein in the placental villous tissues cultured for 4 h under hypoxic condition(2% O₂)were detected in two groups.The expressions of HIF-1α increased in the placentae of pregnant women with ICP(P<0.05).With the O₂ concentration recovered, the high O₂ concentration(21% O₂)decreased the expression HIF-1α in 15 min(P<0.05)in two groups.②The expression of HIF-2α was highly increased under hypoxia condition than that under normal concentration O₂(P<0.01); 21 % O₂ decreased the expression HIF-2α in 15 min(P<0.01)in normal group.But no significant difference was revealed in the change of the HIF-2α in ICP(P > 0.05).

Conclusions

Our research suggested that as a sensitive index, high increase of HIF-la may play an important pole in adapting hypoxia and the abnormal change of HIF-2α may also bring bad pregnancy outcome in ICP under hypoxic conditions.

Key words: intrahepatic cholestasis of pregnancy(ICP), hypoxia-inducible transcription factor

图1　缺氧时ICP胎盘HIF-1α的表达　SP×200

图2　缺氧时ICP胎盘HIF-2α的表达　SP×200

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