Study on the expression of mRNA for MCP-1 in cerebral tissue of newborn rats with experimental hypoxic-ischemic brain damage

doi:10.3877/cma.j.issn.1673-5250.2006.01.111

Objective

To observe the expression of mRNA for the MCP-1 in cerebral tissue after experimental hypoxic-ischemic brain damage(HIBD)in newborn rats.

Methods

The HIBD model of SD rat was set up by ligating the right carotid artery and exposing the animals to 8% oxygen for 2. 5 hours. The dynamic changes of expression of MCP-1 mRNA were studied by using quantitative, real-time, fluorogenic PCR(TaqMan)assay.

Results

Peaking expression of MCP-1 mRNA was shown at 6 hours after HIBD and the level of MCP-1 mRNA in the hypoxic-ischemic(HI)cerebral hemisphere was significantly higher than that of the control group(P<0. 05). The level of MCP-1 mRNA in the HI cerebral hemisphere remained greater than that of the control group even at 12 hours after HIBD(P< 0. 05). The expression of MCP-1 mRNA declined at 24〜72h after HIBD in the HI cerebral hemisphere and there was no difference compared with that in the control group(P>0. 05).

Conclusion

It is suggested that as inflammatory mediators, the MCP-1 expression after HIBD may play an important role in the mechanism of brain damage.

Key words: monocyte chemoattractant protein-1(MCP-1), hypoxic-ischemic brain damage, neonatal, rat

	1　Perttu J，Lindsberg A，Grau J，et al. Inflammation and infections as risk factors for ischemic. Stroke, 2003，34(10)：2518-2532.
	2　姚裕家，李炜如，李念等.地塞米松在新生猪缺氧缺血性脑损伤中的防治作用.中华儿科杂志，1994, 32(4)：201-202.
	3　Galasso J，Miller M，Cowell M，et al. Acute Excitotoxic injury induces expression of monocyte chemoattractant protein-1 and its receptor, ccr2 in neonatal rat brain. Exper Neurol，2000，165(2): 295-305.
	4　Adrian B，Rudy B，Simone B，et al. Chemokines and their receptors in the central nervous system. Fronti Neuroendocrinol，2001，22(3):147-184.
	5　Chen Y, John M，Ruetzler C，et al. Overexpression of monocyte chemoattractant protein 1 in the brain exacerbates ischemic brain injury andis associated with recruitment of inflammatory cells. Cerebral Blood Flow Metab, 2003，23(6)：748-755.

[1]	Hui Luo, Ye Fang. Application of quality control circles in improving detection rate of thyroid nodules by fine-needle aspiration[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2024, 21(10): 972-977.
[2]	Sirui Liu, Chenyang Zhao, Rui Zhang, Yixiu Zhang, Meng Yang. Comprehensive evaluation of hemodynamic parameters at different segments of the uterine artery in pregnant rats using Doppler ultrasonography[J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2024, 21(09): 877-883.
[3]	Liuqing Li, Xiaoxiang Chen, Chengyu Lv. Comparison of total laparoscopic and laparoscopic assisted radical gastrectomy in the treatment of advanced gastric cancer in the near and medium term[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 23-26.
[4]	Shijun Liu, Jie Ma, Lujing Shi. Near-and medium-term follow-up study of complete mesangectomy plus standard D2 radical resection for advanced gastric cancer[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 27-30.
[5]	Lixia Zhao, Chunxia Wang, Yifeng Chen, Dongping Hu, Weisheng Zhang, Tao Wang, Honglai Zhang. Effects of visceral obesity on early complications after laparoscopic radical resection for rectal cancer[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 35-39.
[6]	Huazhi Li, Guang Cao, Diangang Liu, Yajing Zhang. Clinical comparison of descending hepatectomy with different approaches for primary hepatocellular carcinoma[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 52-55.
[7]	Xiaowei Chang, Yu Cai, Zhiyong Zhao, Wei Zhang. Efficacy and safety analysis of high intensity focused ultrasound ablation combined with hepatic arterial chemoembolization in the treatment of primary hepatocellular carcinoma[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 56-59.
[8]	Bo Li, Pengbo Jia, Dong Li, Xiaoqing Li. Effect of ERCP and LCBDE on acute severe cholangitis secondary to choledocholithiasis[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 60-63.
[9]	Tianwei Xie, Yufan Pang, Li Wu. Effect of different ultrasound-guided ablation on the volume reduction rate and recurrence rate of benign thyroid nodules[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 80-83.
[10]	Lu Wang, Lijun Zhou. Effects of different anastomotic methods in total laparoscopic distal subtotal gastrectomy on gastric function recovery and complication rate in patients with gastric cancer[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 92-95.
[11]	Lian Sun, Hongping Ma, Wenying Wu. Surgical management of patients with locally advanced thyroid cancer[J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 112-114.
[12]	Ying Zhang, Xin Zhao, Jiamei Chen, Yan Li. Impact of preoperative chemotherapy on prognosis of pseudomyxoma peritonei treated by cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy：a meta-analysis[J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(09): 826-835.
[13]	Mingmin Chen, Jingjing Ren. Investigation of narrative ability of general practitioners[J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(09): 836-840.
[14]	Qi Liu, Shoukai Wang, Shuai Wang, Yuqing Su, Zhuang Ma, Haijun Chen, Pilei Si. Advances in research of intratumoral microbiome in breast cancer[J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(09): 841-845.
[15]	Jingming Wang, Lei Wang, Xiaoduo Xu, Wenqiang Xing, Zhaoyan Zhang, Weimin Huang. Advances in research of paravertebral muscles of the lumbar spine[J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(09): 846-852.

Viewed

Full text

Abstract

Please choose a citation manager

Content to export