切换至 "中华医学电子期刊资源库"

中华妇幼临床医学杂志(电子版) ›› 2016, Vol. 12 ›› Issue (01) : 96 -99. doi: 10.3877/cma.j.issn.1673-5250.2016.01.018

所属专题: 文献

综述

小分子干扰RNA干扰相关基因表达对卵巢癌化疗敏感性影响的研究进展
李冉红1, 陈晨1, 刘辉1,*,*()   
  1. 1. 610041 成都,四川大学华西第二医院妇产科
  • 收稿日期:2015-08-09 修回日期:2015-12-09 出版日期:2016-02-01
  • 通信作者: 刘辉

Research progress of related gene expression interfered by small interfering RNA in drug-sensibility of ovarian cancer chemotherapy

Ranhong Li1, Chen Chen1, Hui Liu1()   

  1. 1. Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2015-08-09 Revised:2015-12-09 Published:2016-02-01
  • Corresponding author: Hui Liu
  • About author:
    Corresponding author: Liu Hui, Email:
引用本文:

李冉红, 陈晨, 刘辉. 小分子干扰RNA干扰相关基因表达对卵巢癌化疗敏感性影响的研究进展[J]. 中华妇幼临床医学杂志(电子版), 2016, 12(01): 96-99.

Ranhong Li, Chen Chen, Hui Liu. Research progress of related gene expression interfered by small interfering RNA in drug-sensibility of ovarian cancer chemotherapy[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2016, 12(01): 96-99.

卵巢癌是女性生殖系统常见3大恶性肿瘤之一,导致的患者病死率居妇科恶性肿瘤首位,严重威胁女性的健康与生命。目前卵巢癌以手术和化疗为主要治疗方法,而肿瘤对化疗药物产生耐药,是导致治疗失败的重要原因之一。因此,研究卵巢癌细胞的耐药性逆转作用,对提高临床治疗效果意义重大。而小分子干扰RNA(siRNA)是由双链RNA在多种酶共同作用下产生的小片段双链RNA,可与同源的靶mRNA互补结合,特异性降解靶mRNA。因此,通过设计针对性的siRNA干扰与肿瘤发生、发展相关的基因,如Id1、STAT3、MDR1、BAF57及CXCR4等表达,可提高卵巢癌细胞对化疗药物的敏感性,为卵巢癌细胞耐药性逆转研究提供初步理论依据。

Ovarian cancer is one of the three most common and lethal malignance in reproductive system of women, the mortality of which has become the highest in gynecological cancer.Ovarian cancer has been severely threatened the health and life of females.At present, surgery and chemotherapeutic have become the main therapy for ovarian cancer, however, the drug-resistance of tumors has been the important reason for the treatment failure. So study on the reversion of drug- resistance in ovarian cancer cell is significant for improving the effect of clinical treatment of ovarian cancer.Small interfering RNA(siRNA) is small fragments of double-stranded RNA produced jointly by a variety of enzymes, which can combine with homologous target mRNA complementarily and degraded target mRNA specifically.So, designing the specific siRNA to interference with expression of genes which related with the occurrence and development of tumors, such as Id1, STAT3, MDR1, BAF57 and CXCR4, etc., can improve the sensitivity of ovarian cancer cells to chemotherapy drugs, which provides the preliminary theoretical basis for research of ovarian cancer cells resistance reversal.

1
McGuire WP 3rd, Markman M.Primary ovarian cancer chemotherapy:current standards of care[J].Br J Cancer, 2003, 89(suppl 3):s3-s8.
2
Averick SE, Paredes E, Dey SK, et al.Autotransfecting short interfering RNA through facile covalent polymer escorts[J].J Am Chem Soc, 2013, 135(34):12508-12511.
3
Aranha MM, Solá S, Low WC, et al. Caspases and P53 modulate FOXO3A/Id1 signaling during mouse neural stem cell differentiation[J].J Cell Biochem, 2009, 107(4):748-758.
4
Lyden D, Young AZ, Zagzag D, et al.Id1 and Id3 are required for neurogenesis, angiogenesis and vascularization of tumour xenografts[J].Nature, 1999, 401(6754):670-677.
5
Su Y, Gao L, Teng L, et al.Id1 enhances human ovarian cancer endothelial progenitor cell angiogenesis via PI3K/Akt and NF-κB/MMP-2 signaling pathways[J].J Transl Med, 2013, 11:132.
6
Maw MK, Fujimoto J, Tamaya T.Overexpression of inhibitor of DNA-binding (ID)-1 protein related to angiogenesis in tumor advancement of ovarian cancers[J]. BMC Cancer, 2009, 9:430.
7
史洵玮,何海林,王霞,等.siRNA干扰Id1表达对卵巢癌化疗耐药性的影响[J].实用妇产科杂志,2011,27(12):897-900.
8
Pan Y, Zhang L, Zhang X, et al.Synergistic effects of eukaryotic co-expression plasmid-based STAT3-specific siRNA and LKB1 on ovarian cancer in vitro and in vivo[J].Oncol Rep, 2015, 33(2):774-782.
9
Yang X, Iyer AK, Singh A, et al.Cluster of differentiation 44 targeted hyaluronic acid based nanoparticles for mdr1 siRNA delivery to overcome drug resistance in ovarian cancer[J].Pharm Res, 2015, 32(6):2097-2109.
10
Neigeborn L, Carlson M. Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae[J]. Genetics, 1984, 108(4):845-858.
11
Stern M, Jensen R, Herskowitz I.Five SWI genes are required for expression of the HO gene in yeast[J].J Mol Biol, 1984, 178(4):853-868.
12
Pazin MJ, Kadonaga JT.SWI2/SNF2 and related proteins:ATP-driven motors that disrupt protein-DNA interactions?[J].Cell, 1997, 88(6):737-740.
13
Yamaguchi T, Kurita T, Nishio K, et al. Expression of BAF57 in ovarian cancer cells and drug sensitivity[J].Cancer Sci, 2015, 106(4):359-366.
14
Barbero S, Bonavia R Bajetto A, et al.Stromal cell-derived factor 1alpha stimulates human glioblastoma cell growth through the activation of both extracellular signal-regulated kinase 1/2 and Akt[J].Cancer Res, 2003, 63(8):1969-1974.
15
Liu W, Wang Y, Wang H, et al.Anticancer effects of chemokine receptor 4(CXCR4) gene silenced by CXCR4-siRNA in nude mice model of ovarian cancer[J].Cell Biochem Biophys, 2014, 70(3):1893-1900.
16
Zhao LJ, Xu H, Qu JW, et al. Modulation of drug resistance in ovarian cancer cells by inhibition of protein kinase C-alpha (PKC-α) with small interference RNA (siRNA) agents[J].Asian Pac J Cancer Prev, 2012, 13(8):3631-3636.
[1] 王雪菲, 海琳悦, 李立方, 肖春花. Luminal A型乳腺癌的内分泌治疗与化疗[J]. 中华乳腺病杂志(电子版), 2023, 17(05): 294-300.
[2] 林昌盛, 战军, 肖雪. 上皮性卵巢癌患者诊疗中基因检测及分子靶向药物治疗[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(05): 505-510.
[3] 王璐, 樊杨. 子宫内膜癌相关生物标志物研究现状[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(05): 511-516.
[4] 代雯荣, 赵丽娟, 李智慧. 细胞外囊泡对胚胎着床影响的研究进展[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(05): 616-620.
[5] 陈荟竹, 郭应坤, 汪昕蓉, 宁刚, 陈锡建. 上皮性卵巢癌"二元论模型"的分子生物学研究现状[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(04): 394-402.
[6] 刘星辰, 刘娟, 魏宝宝, 刘洁, 刘辉. XIAP与XAF1异常表达与卵巢癌的相关性分析[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(04): 419-427.
[7] 陈永胜, 朱健, 王军, 张永辉, 徐源佑, 丁璐璐, 陈建国, 樊健, 姚海蓉. 江苏省启东市育龄女性恶性肿瘤患者生存率变化趋势分析[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(03): 363-372.
[8] 涂家金, 廖武强, 刘金晶, 涂志鹏, 毛远桂. 严重烧伤患者鲍曼不动杆菌血流感染的危险因素及预后分析[J]. 中华损伤与修复杂志(电子版), 2023, 18(06): 491-497.
[9] 张海金, 王增国, 蔡慧君, 赵炳彤. 2020至2022年西安市儿童医院新生儿细菌感染分布及耐药监测分析[J]. 中华实验和临床感染病杂志(电子版), 2023, 17(04): 222-229.
[10] 李晓阳, 刘柏隆, 周祥福. 大数据及人工智能对女性盆底功能障碍性疾病的诊断及风险预测[J]. 中华腔镜泌尿外科杂志(电子版), 2023, 17(06): 549-552.
[11] 张茜, 刘叶青, 康雪莹, 孙兵兵, 刘岩, 胡丽叶, 周亚茹. 血清铁蛋白与绝经后骨质疏松症的相关性分析[J]. 中华老年骨科与康复电子杂志, 2023, 09(03): 166-171.
[12] 王飞飞, 王光林, 孟泽松, 李保坤, 曹龙飞, 张娟, 周超熙, 丁源一, 王贵英. 敲低IMPDH1对结肠癌SW480、HT29细胞生物功能的影响[J]. 中华临床医师杂志(电子版), 2023, 17(08): 884-890.
[13] 高红琴, 陈晨, 陆瑞科, 王小雨, 张敏, 李少华, 郝梨岚, 黄新程, 关凌耀, 张韵红. 外阴阴道假丝酵母菌病对女性阴道-宫颈菌群的影响研究[J]. 中华临床医师杂志(电子版), 2023, 17(06): 720-725.
[14] 林舒楠, 党文强, 钟天, 梁斯欣, 张磊, 唐晓华, 袁文常. 2017—2021年广东地区基层医疗机构金黄色葡萄球菌临床分离株耐药谱分析[J]. 中华临床实验室管理电子杂志, 2023, 11(03): 139-144,150.
[15] 刘育昕, 王子晗, 张艺馨, 栾永婕, 孟凯. 肾母细胞瘤基因1在卵巢疾病发病机制中的研究进展[J]. 中华诊断学电子杂志, 2023, 11(03): 178-183.
阅读次数
全文


摘要