<i>MIMECAN</i>基因、细胞核因子κB和白细胞介素24与子宫内膜癌的相关性研究

doi:10.3877/cma.j.issn.1673-5250.2015.02.018

目的

探讨MIMECAN基因、细胞核因子(NF)-κB和白细胞介素(IL)-24在子宫内膜癌中的表达及其关系。

方法

选择2008年1月至2013年1月于徐州医学院附属淮安医院妇产科住院治疗的120例子宫内膜癌患者为研究对象，将其纳入子宫内膜癌组(n＝120)，再按照国际妇产科联盟(FIGO)病理学分期标准将其进一步分为4个亚组，分别为Ⅰ期亚组(n＝45)、Ⅱ期亚组(n＝30)、Ⅲ期亚组(n＝24例)和Ⅳ期亚组(n＝21)。选择同期在本院行健康体检的40例正常妇女为对照组(n＝40)。采用酶联免疫吸附测定法(ELISA)检测各组血清MIMECAN基因、NF-κB和IL-24水平。本研究遵循的程序符合徐州医学院附属淮安医院妇产科人体试验委员会所制定的伦理学标准，得到该委员会批准，分组征得受试对象本人的知情同意，并与之签署临床研究知情同意书。

结果

①子宫内膜癌组各亚组患者血清MIMECAN基因、NF-κB和IL-24水平均明显高于对照组，差异均有统计学意义(P<0.05)，且各亚组间两两比较，差异也有统计学意义(P<0.05)。②MIMECAN基因、NF-κB和IL-24与子宫内膜癌严重程度呈正相关关系(r＝0.69，0.73，0.82；P<0.05)。③子宫内膜癌组MIMECAN基因和NF-κB呈正相关关系(r＝0.76，P<0.05)；NF-κB和IL-24也呈正相关关系(r＝0.78, P<0.05)。

结论

MIMECAN基因、NF-κB和IL-24相互作用可能促进子宫内膜癌的发生、发展。

关键词: MIMECAN基因, 细胞核因子κB, 白细胞介素24, 子宫内膜癌

Objective

To explore the expression of MIMECAN gene, cell nuclear factor(NF)-κB, interleukin(IL)-24 and their relationship with endometrial carcinoma.

Methods

From January 2008 to January 2013, a total of 120 hospitalized patients with endometrial carcinoma in Huai'an Affiliated Hospital of Xuzhou Medical College were included in the study as research group(n=120), they were further divided into stageⅠ subgroup(n=45), stageⅡsubgroup (n=30), stage Ⅲsubgroup (n=24) and stage Ⅳ subgroup (n=21) according to the International Federation of Gynecology and Obstetrics(FIGO) pathological stages.Meanwhile, another 40 healthy women were chosen as control group(n=40). The expression of MIMECAN gene, NF-κB, IL-24 levels were detected by enzyme-linked immunosorbent assay(ELISA). The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Affiliated Hospital of Xuzhou Medical College.Informed consent was obtained from each participants.

Results

①The levels of MIMECAN gene, NF-κB, IL-24 in research group were obviously higher than those of control group, and the differences were significant(P<0.05). There also were significant differences among four subgroups in levels of MIMECAN gene, NF-κB, IL-24(P<0.05). ②There were positive correlation between levels of MIMECAN gene, NF-κB, IL-24 and the severity of endometrial carcinoma(r=0.69, 0.73, 0.82; P<0.05). ③The NF-κB was positively correlated with MIMECAN gene and IL-24 in research group(r=0.76, 0.78; P<0.05).

Conclusions

MIMECAN gene, NF-κB, IL-24 interaction might promote the development of endometrial carcinoma.

Key words: MIMECAN gene, Cell nuclear factor κB, Interleukin 24, Endometrial carcinoma

表1 各组患者一般临床资料比较(±s)

Table 1 Comparison of general clinical data among these groups(±s)

组别	例数	年龄(岁)	BMI(kg/m²)	空腹血糖(mmol/L)	血压(mmHg)
子宫内膜癌组	120	59.3±3.8	23.06±1.83	5.78±0.23	(123.91±11.52)/(82.52±6.98)
Ⅰ期亚组	45	56.4±3.3	23.21±2.53	5.80±0.28	(125.21± 9.58)/(80.21±7.62)
Ⅱ期亚组	30	57.2±4.6	22.52±3.76	5.69±0.32	(119.08±11.12)/(79.81±9.15)
Ⅲ期亚组	24	60.23±5.7	21.82±2.87	5.74±0.29	(120.32±13.50)/(82.30±7.05)
Ⅳ期亚组	21	61.0±6.6	23.24±3.36	5.82±0.17	(124.91±12.59)/(81.72±8.01)
对照组	40	58.8±6.5	22.02±3.01	5.71±0.37	(122.50±10.35)/(83.32±8.05)

表1 各组患者一般临床资料比较(±s)

Table 1 Comparison of general clinical data among these groups(±s)

组别	例数	年龄(岁)	BMI(kg/m²)	空腹血糖(mmol/L)	血压(mmHg)
子宫内膜癌组	120	59.3±3.8	23.06±1.83	5.78±0.23	(123.91±11.52)/(82.52±6.98)
Ⅰ期亚组	45	56.4±3.3	23.21±2.53	5.80±0.28	(125.21± 9.58)/(80.21±7.62)
Ⅱ期亚组	30	57.2±4.6	22.52±3.76	5.69±0.32	(119.08±11.12)/(79.81±9.15)
Ⅲ期亚组	24	60.23±5.7	21.82±2.87	5.74±0.29	(120.32±13.50)/(82.30±7.05)
Ⅳ期亚组	21	61.0±6.6	23.24±3.36	5.82±0.17	(124.91±12.59)/(81.72±8.01)
对照组	40	58.8±6.5	22.02±3.01	5.71±0.37	(122.50±10.35)/(83.32±8.05)

表2 各组血清MIMECAN基因、NF-κB和IL-24水平比较(mg/L，±s)

Table 2 Comparison of serum MIMECAN gene，NF-κB and IL-24 levels among these groups(mg/L，±s)

组别		例数	MIMECAN基因	NF-κB	IL-24
子宫内膜癌组		120	18.09±3.86	26.83±7.69	0.89±0.24
?	Ⅰ期亚组	45	11.72±2.37^a	22.21±3.43^b	0.68±0.11^c
?	Ⅱ期亚组	30	14.91±1.81^ad	23.62±4.35^bg	0.81±0.14^cj
?	Ⅲ期亚组	24	17.36±2.53^ade	27.26±6.60^bgh	0.91±0.12^cjk
?	Ⅳ期亚组	21	20.65±2.81^ade	31.32±5.47^bgh	1.13±0.10^cjk
对照组		40	4.78±0.63^f	18.73±4.31ⁱ	0.58±0.13^l
F值		?	416.93	631.97	310.62
P值		?	<0.05	<0.05	<0.05

表2 各组血清MIMECAN基因、NF-κB和IL-24水平比较(mg/L，±s)

Table 2 Comparison of serum MIMECAN gene，NF-κB and IL-24 levels among these groups(mg/L，±s)

组别		例数	MIMECAN基因	NF-κB	IL-24
子宫内膜癌组		120	18.09±3.86	26.83±7.69	0.89±0.24
?	Ⅰ期亚组	45	11.72±2.37^a	22.21±3.43^b	0.68±0.11^c
?	Ⅱ期亚组	30	14.91±1.81^ad	23.62±4.35^bg	0.81±0.14^cj
?	Ⅲ期亚组	24	17.36±2.53^ade	27.26±6.60^bgh	0.91±0.12^cjk
?	Ⅳ期亚组	21	20.65±2.81^ade	31.32±5.47^bgh	1.13±0.10^cjk
对照组		40	4.78±0.63^f	18.73±4.31ⁱ	0.58±0.13^l
F值		?	416.93	631.97	310.62
P值		?	<0.05	<0.05	<0.05

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Relationship of MIMECAN gene, cell nuclear factor-κB and interleukin-24 with endometrial cancer

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Relationship of MIMECAN gene, cell nuclear factor-κB and interleukin-24 with endometrial cancer