趋化因子CXCL12在子宫腺肌病组织中的表达及意义

doi:10.3877/cma.j.issn.1673-5250.2014.03.013

目的

探讨趋化因子CXCL12在子宫腺肌病发病中的作用。

方法

选择2010年2月至2011年2月在济南军区总医院行全、次全子宫切除术或病灶切除术，经病理学确诊的36例子宫腺肌病患者术前、术中的取材标本为研究对象，并根据取材部位不同将其分为在位内膜组(n＝36)、异位病灶组(n＝36)和病灶周围组织组(n＝36)。选择同期住院手术治疗的33例子宫肌瘤患者术中取材标本为对照，按照取材部位不同，将其分别纳入对照内膜组(n＝33)和对照肌层组(n＝33)(本研究遵循的程序符合济南军区总医院人体试验委员会所制定的伦理学标准，得到该委员会批准，分组征得受试对象本人的知情同意，与之签署临床研究知情同意书)。子宫腺肌病患者和子宫肌瘤患者年龄等一般资料比较，差异无统计学意义(P>0.05)。对各组CXCL12蛋白表达和CXCL12 mRNA的含量进行比较及相关性分析。

结果

①异位病灶组CXCL12蛋白表达显著高于在位内膜组和对照内膜组，前者分别与后二者比较，差异有统计学意义(P<0.05)，在位内膜组CXCL12蛋白表达与对照内膜组比较，差异亦有统计学意义(P<0.05)。②在位内膜组、病灶周围组织组和异位病灶组CXCL12 mRNA含量均高于对照内膜组，前三者分别与后者比较，差异均有统计学意义(P<0.05)；异位病灶组CXCL12 mRNA含量分别与在位内膜组和病灶周围组织组比较，差异也有统计学意义(P<0.05)。③Pearson相关分析结果显示，在位内膜组CXCL12蛋白表达与异位病灶组具有相关关系(r＝0.780，P＝0.000)；在位内膜组CXCL12 mRNA含量与病灶周围组织组、异位病灶组均呈正相关关系(r＝0.499和0.461; P＝0.002和0.005)；病灶周围组织组CXCL12 mRNA含量与异位病灶组呈正相关关系(r＝0.679，P＝0.000)。

结论

CXCL12在子宫腺肌病发生、发展过程中起一定作用，其可能通过诱导内膜细胞异位发挥致病作用。

关键词: 子宫内膜异位症, 趋化因子CXCL12, 发病

Objective

To investigate the significance of chemokine CXCL12 in the pathogenesis of uterine adenomyosis.

Methods

From February 2010 to February 2011, a total of 36 women with uterine adenomyosis were included in the study, and their pathological samples were divided into ectopic lesions group(n=36), surrounding tissues group(n=36) and eutopic endometrium group(n=36). Meanwhile pathological samples from other 33 uterine fibroids patients were included into control endometrium group(n=33) and control myometrium group(n=33). Expression of CXCL12 protein and CXCL12 mRNA content were detected. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Jinan Millitary General Hospital.Informed consent was obtained from each participants.

Results

①CXCL12 protein expression in ectopic lesions group was significantly higher than that of eutopic endometrium group and control endometrium group(P<0.05), and CXCL12 protein expression in eutopic endometrium group was significantly higher than that of control endometrium group(P<0.05). ②There had significant differences in CXCL12 mRNA content between eutopic endometrium group, surrounding tissues group, ectopic lesions group and control endometrium group, respectively(P<0.05). Compared with eutopic endometrium group and surrounding tissues group, CXCL12 mRNA content of ectopic lesions group had remarkably increased(P<0.05). ③CXCL12 protein expression in eutopic endometrium group and ectopic lesions group had significant correlation (r=0.78, P<0.05). CXCL12 mRNA content in eutopic endometrium group had positive correlation with ectopic lesions group and surrounding tissues group(r=0.499, 0.461; P=0.002, 0.005). There had significant correlation between ectopic lesions group and surrounding tissues group (r=0.679, P=0.000).

Conclusions

CXCL12 may be play an important role in pathogenesis of uterine adenomyosis by inducing ectopic endometrial cells.

Key words: Endometriosis, Chemokine CXCL12, Pathogenesis

表1 3组CXCL12蛋白表达比较(±s)

Table 1 Comparison of CXCL12 protein expression among three groups (±s)

组别	n	CXCL12	t	P
异位病灶组	36	9.78±2.02	11.576^a	0.000^a
在位内膜组	36	4.33±2.12	13.968^b	0.000^b
对照内膜组	33	3.06±1.82	2.647^c	0.009^c

表1 3组CXCL12蛋白表达比较(±s)

Table 1 Comparison of CXCL12 protein expression among three groups (±s)

组别	n	CXCL12	t	P
异位病灶组	36	9.78±2.02	11.576^a	0.000^a
在位内膜组	36	4.33±2.12	13.968^b	0.000^b
对照内膜组	33	3.06±1.82	2.647^c	0.009^c

图1 CXCL12蛋白在异位病灶中的表达(HE, ×400)

Figure 1 Expression of CXCL12 protein in ectopic lesion(HE, ×400)

图2 CXCL12蛋白在位病灶中的表达(HE, ×400)

Figure 2 Expression of CXCL12 protein in eutopic endometrium(HE, ×400)

图3 CXCL12蛋白在对照内膜病灶中的表达(HE, ×400)

Figure 3 Expression of CXCL12 protein in control endometrium(HE, ×400)

表2 5组CXCL12 mRNA含量比较(±s)

Table 2 Comparison of CXCL12 mRNA content among five groups(±s)

组别	n	ΔCt	t	P
异位病灶组	36	1.26±0.76	－2.784^a	0.006^a
病灶周围组织组	36	1.82±1.15	－2.442^b	0.016^b
在位内膜组	36	1.85±1.30	－4.990^c	0.000^c
对照内膜组	33	2.40±0.77	－2.602^d	0.011^d
对照肌层组	33	2.34±0.46	－2.552^e	0.012^e

表2 5组CXCL12 mRNA含量比较(±s)

Table 2 Comparison of CXCL12 mRNA content among five groups(±s)

组别	n	ΔCt	t	P
异位病灶组	36	1.26±0.76	－2.784^a	0.006^a
病灶周围组织组	36	1.82±1.15	－2.442^b	0.016^b
在位内膜组	36	1.85±1.30	－4.990^c	0.000^c
对照内膜组	33	2.40±0.77	－2.602^d	0.011^d
对照肌层组	33	2.34±0.46	－2.552^e	0.012^e

1	Yu L, Cecil J, Peng SB, et al.Identification and expression of novel isoforms of human stromal cell-derived factor[J].Gene, 2006, 374(7):174-179.
2	白红，赵丽嫣．子宫腺肌病病因与发病机制研究进展[J]．中国妇产科临床，2003，4(6)：61-64.
3	Laing KJ, Secombes CJ.Chemokines[J].Dev Comp Immunol, 2004, 28(5):443.
4	Lortat-Jacob H.The molecular basis and functional implications of chemokine interactions with heparan sulphate[J].Cur Opin Struct Biol, 2009, 19(5):543-548.
5	Laird SM, Widdowson R, El-Sheikhi M, et al.Expression of CXCL12 and CXCR4 in human endometrium; effects of CXCL12 on MMP production by human endometrial cells[J].Hum Reprod, 2011, 26(5):1144-1152.
6	Muller A, Homey B, Soto H, et al.Involvement of chemokine receptors in breast cancer metastasis[J].Nature, 2001, 410(6824):50-56.
7	李芳，朱怀仕．卵巢癌趋化因子受表达及意义[J]．济宁医学院学报，2003，26(3)：1-3.
8	Kollmar O, Rupertus K, Scheuer C, et al.Stromal cell-derived factor-1 promotes cell Migration and tumor growth of colorectal metastasis[J].Neoplasia, 2007, 9(10):862-870.
9	Guleng B, Tateishi K, Ohta M, et al.Blockade of the stromal cell-derived factor-1/CXCR4 axis attenuates in vivo tumor growth by inhibiting angiogenesis in a vascular endothelial growth factor-independent manner[J].Cancer Res, 2005, 65(13):5864-5871.
10	韩燕华，周应芳，郑淑蓉．子宫腺肌病患者子宫各部位血管形态的研究[J]．中国实用妇科与产科杂志，2002，18(10)：605-607.
11	Phillips RJ, Burdick MD, Lutz M, et al.The stromal derived factor-1/CXCL12-CXC chemokine receptor 4 biological axis in non-small cell lung cancer metastases[J].Am J Respir Crit Care Med, 2003, 167(12):1676.

[1]	曾敬, 吴冬冬, 邵明, 范震波, 王治国, 刘培谊, 兰海峰. 高龄髋部骨折患者不同手术时机的围手术期疗效评估[J/OL]. 中华关节外科杂志(电子版), 2024, 18(04): 445-449.
[2]	高旭, 李若凡, 孙立新, 刘佐军, 田广健. Miles手术中预置腹膜前补片预防造口旁疝的效果及安全性[J/OL]. 中华疝和腹壁外科杂志(电子版), 2024, 18(03): 266-272.
[3]	刘璐璐, 何羽. 慢性阻塞性肺病患者睡眠障碍的研究进展[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(05): 836-839.
[4]	张蕾, 彭超, 周应芳. 直肠阴道隔子宫内膜异位症腹腔镜手术技巧[J/OL]. 中华腔镜外科杂志(电子版), 2024, 17(05): 257-261.
[5]	胡启明, 鄢潇, 尤志学, 黄骁昊. 经瘢痕处单孔腹腔镜下切除多病灶腹壁子宫内膜异位症[J/OL]. 中华腔镜外科杂志(电子版), 2024, 17(05): 314-317.
[6]	林科灿, 罗柳平. 肝胰十二指肠切除术在胆囊癌和胆管癌应用中的关键问题[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 774-778.
[7]	芦煜, 李振宇, 吴承东, 周仲伍. 肛周子宫内膜异位症一例报告[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(05): 431-434.
[8]	杨明, 张金珠, 王锡山. 全国肿瘤登记中心发布的2013年至2022年结直肠癌流行数据趋势解读[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(03): 177-181.
[9]	赵静, 张嘉欣, 高言, 谢席胜. 微小病变肾病的发病机制及治疗研究进展[J/OL]. 中华肾病研究电子杂志, 2024, 13(04): 207-212.
[10]	贾红艳, 王丹, 张冉冉, 马茜, 焦永红. 基于全外显子组测序探寻Möbius综合征发病机制的遗传学研究[J/OL]. 中华眼科医学杂志(电子版), 2024, 14(03): 146-154.
[11]	安亚楠, 王端然, 郭甜甜, 武希润. 幽门螺杆菌阴性胃黏膜相关淋巴组织淋巴瘤的研究进展[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(03): 268-274.
[12]	刘琦, 王守凯, 王帅, 苏雨晴, 马壮, 陈海军, 司丕蕾. 乳腺癌肿瘤内微生物组的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(09): 841-845.
[13]	洪凡, 陈敦金, 傅洋, 梁新月, 吴毅, 王晓怡. 体外受精-胚胎移植妊娠合并前置胎盘临床研究[J/OL]. 中华产科急救电子杂志, 2024, 13(03): 176-182.
[14]	朱镭, 朱庆义. 金氏菌属：引起婴幼儿侵袭性传染病的新发病原体[J/OL]. 中华临床实验室管理电子杂志, 2024, 12(04): 229-237.
[15]	厉若男, 宋进, 王玉忠. 带状疱疹后神经痛的发病机制和诊治研究进展[J/OL]. 中华诊断学电子杂志, 2024, 12(03): 199-205.

阅读次数

全文

摘要

选择文件类型/文献管理软件名称

选择包含的内容

Expression and Significance of Chemokine CXCL12 in Uterine Adenomyosis

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

Expression and Significance of Chemokine CXCL12 in Uterine Adenomyosis