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中华妇幼临床医学杂志(电子版) ›› 2013, Vol. 09 ›› Issue (02) : 189 -192. doi: 10.3877/cma.j.issn.1673-5250.2013.02.013

所属专题: 文献

论著

CA125,CA19-9在子宫内膜癌组织学和血清学中表达水平的差异性及相关性研究
杨岚1,*(), 郭友逢2,*(), 王丽峰1, 王娟1, 文清华1   
  1. 1. 524400 广东廉江,广东医学院附属廉江医院
    2. 中山大学附属第二医院病理科
  • 收稿日期:2012-11-18 修回日期:2013-02-14 出版日期:2013-04-01
  • 通信作者: 杨岚, 郭友逢

Correlation and Differences Between Tissues and Serum Level of CA125 and CA19-9 in Endometrial Cancer

Lan YANG1(), You-feng GUO2(), Li-feng WANG1, Juan WANG1, Qing-hua WEN1   

  1. 1. Department of Gynecology and Obstetrics, Affiliated Lianjiang Hospital of Guangdong Medical College, Lianjiang 524400, Guangdong Province, China
  • Received:2012-11-18 Revised:2013-02-14 Published:2013-04-01
  • Corresponding author: Lan YANG, You-feng GUO
  • About author:
    (Corresponding author: YANG Lan, Email: )
引用本文:

杨岚, 郭友逢, 王丽峰, 王娟, 文清华. CA125,CA19-9在子宫内膜癌组织学和血清学中表达水平的差异性及相关性研究[J/OL]. 中华妇幼临床医学杂志(电子版), 2013, 09(02): 189-192.

Lan YANG, You-feng GUO, Li-feng WANG, Juan WANG, Qing-hua WEN. Correlation and Differences Between Tissues and Serum Level of CA125 and CA19-9 in Endometrial Cancer[J/OL]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2013, 09(02): 189-192.

目的

探讨CA125,CA19-9在子宫内膜癌组织学和血清学中表达水平的差异性及相关性。

方法

选择2008年4月至2012年4月于广东医学院附属廉江医院就诊并诊断为子宫内膜癌患者65例为研究对象,同时选取正常子宫内膜者30例纳入对照组及子宫内膜异位症(EMs)患者40例纳入EMs组。采用ELISA法检测3组患者血清CA125和CA19-9水平,并采用免疫组化和Western blot法检查3组患者组织CA125和CA19-9表达水平,分析CA125和CA19-9在子宫内膜癌血清学和组织学中表达水平的相关性(本研究遵循的程序符合广东医学院附属廉江医院人体试验委员会制定的伦理学标准,得到该委员会批准,分组征得受试对象的知情同意,并与之签署临床研究知情同意书)。

结果

Western blot、免疫组化及ELISA法检测结果均显示,CA125,CA19-9水平在子宫内膜癌组中表达最高,显著高于对照组和EMs组,组间比较,差异有统计学意义(P<0.05),此外,CA125,CA19-9在EMs组水平亦高于正常组,两组比较,差异有统计学意义(P<0.05)。子宫内膜癌组患者血清CA125和CA19-9与其组织学表达水平具有相关性(r=1.12,P<0.05;r=2.04,P<0.05)。

结论

CA125和CA19-9在子宫内膜癌中高表达,且血清学水平与组织学水平具有正相关性,具有重要的诊断价值。

Objective

To discuss the expression and relationship of the tissue and serum level of CA125 and CA19-9 in endometrial cancer.

Methods

From April 2008 to April 2012, sixty-five cases of endometrial cancer were selected into this study (endometrial cancer group). Meanwhile, thirty cases of normal endometria were included into control group and 40 cases of endometriosis (EMs) were selected as EMs group. The serum levels of CA125 and CA19-9 were detected by ELISA, and the histological expression level of CA125 and CA19-9 were detected by immunohistochemistry and Western blot. The correlation of CA125 and CA19-9 were analyzed. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Affiliated Lianjiang Hospital of Guangdong Medical College. Informed consent was obtained from all participates.

Results

The expression of CA125 and CA19-9 in endometrial cancer group were significantly higher than those in EMs group and control group (P<0.05). The level of CA125 and CA19-9 in EMs group were significantly higher than those of control group (P<0.05). The serum level of CA125 and CA19-9 were significantly related with histological expression (r=1.12, P<0.05; r=2.04, P<0.05).

Conclusions

CA125 and CA19-9 have high expression in endometrial cancer, and they have positive correlation between serum level and histology level, which have important diagnostic value.

表1 3组患者血清CA125和CA19-9水平及其阳性率比较(±s)
Table 1 Comparison of serum CA125 and CA19-9 levels and positive rates among three groups (±s)
图1 3组HE形态及CA125和CA19-9免疫组化染色(HE×100,免疫组化×100,A:正常子宫内膜,B:EMs,C:子宫内膜癌)
Figure 1 The observation of HE morphological and immunohistochemical staining of CA125 and CA19-9 in three groups (A: Normal endometrial, B: Endometriosis, C: Endometrial cancer)
表2 3组标本的免疫组化结果的CA125和CA19-9阳性率比较[n(%)]
Table 2 Comparison of positive rates of immunohistochemistry of CA125 and CA19-9 among three groups[n(%)]
图2 Western blot检测CA125和CA19-9的表达(1:子宫内膜癌组,2:EMs组,3:正常组)
Figure 2 The expression of CA125 and CA19-9 in the three groups detected by Western blot (A: Endometrial cancer, B: Endometriosis, C: Normal endometrial)
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