切换至 "中华医学电子期刊资源库"

中华妇幼临床医学杂志(电子版) ›› 2013, Vol. 09 ›› Issue (02) : 165 -168. doi: 10.3877/cma.j.issn.1673-5250.2013.02.008

所属专题: 文献

论著

CD4+CD25+调节性T细胞及Foxp3在儿童传染性单核细胞增多症发病中的作用
王强1,*,*(), 王佐凤2, 董巍1, 房俊1   
  1. 1. 610072 成都,四川省人民医院儿科
    2. 攀枝花市中心医院
  • 收稿日期:2013-02-18 修回日期:2013-03-20 出版日期:2013-04-01
  • 通信作者: 王强

The Role of Foxp3, CD4+CD25+ Regulatory T Cells on the Pathogenesis of Infectious Mononucleosis in Children

Qiang WANG1(), Zhuo-feng WANG2, Wei DONG1, Jun FANG1   

  1. 1. Department of Pediatrics, Sichuan Province People's Hospital, Chengdu 610072, Sichuan Province, China
  • Received:2013-02-18 Revised:2013-03-20 Published:2013-04-01
  • Corresponding author: Qiang WANG
  • About author:
    (Corresponding author: WANG Qiang, Email: )
引用本文:

王强, 王佐凤, 董巍, 房俊. CD4+CD25+调节性T细胞及Foxp3在儿童传染性单核细胞增多症发病中的作用[J/OL]. 中华妇幼临床医学杂志(电子版), 2013, 09(02): 165-168.

Qiang WANG, Zhuo-feng WANG, Wei DONG, Jun FANG. The Role of Foxp3, CD4+CD25+ Regulatory T Cells on the Pathogenesis of Infectious Mononucleosis in Children[J/OL]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2013, 09(02): 165-168.

目的

探讨CD4+CD25+调节性T细胞(Treg)及其转录因子Foxp3在儿童传染性单核细胞增多症(IM)发病中的作用。

方法

选择2010年4月至2011年1月于四川省人民医院诊治的35例IM患儿的外周静脉血标本(每例患儿分别采集2个)为研究对象,并按照标本采集时期分为IM急性期组(n=35)和IM恢复期组(n=35)。选择同期于本院儿童保健门诊进行常规体检的35例健康儿童的外周静脉血标本(每例儿童采集1个)为对照组(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象监护人的知情同意,并与之签署临床研究知情同意书)。分别检测3组血样标本中CD3+,CD3+CD4+,CD3+CD8+,CD4+CD25+ Treg表达率及Foxp3 mRNA水平,并进行统计学分析。IM患儿与健康儿童的年龄及性别分布比较,差异无统计学意义(P>0.05)。

结果

IM急性期组外周血CD3+,CD3+CD4+,CD3+CD8+,CD4+/CD8+和CD4+CD25+ Treg测定结果分别与IM恢复期组和对照组比较,差异有统计学意义(P<0.01);IM急性期组Foxp3 mRNA表达水平显著降低,分别与IM恢复期组和对照组比较,差异也有统计学意义(P<0.01),IM恢复期组与对照组比较,差异无统计学意义(P>0.05)。IM急性期组CD4+CD25+ Treg与Foxp3 mRNA表达呈正相关关系(r=0.823,P<0.05)。

结论

IM急性期存在明显免疫失衡,即CD3+CD8+明显增高,CD3+CD4+及CD4+/CD8+明显降低,其免疫失衡原因可能是由于CD4+CD25+ Treg数量降低及其转录因子Foxp3表达下调导致的免疫抑制功能不足所致。

Objective

To investigate the role of CD4+CD25+ regulatory T cells(Treg) and transcription factor Foxp3 on the pathogenesis of infectious mononucleosis(IM) in children.

Methods

From April 2010 to January 2011, 70 blood samples from 35 IM children(2 samples each person) were included into this study, and divided into IM acute stage group(n=35) and IM recovery stage group(n=35) according to the different disease periods.Meanwhile, 35 blood samples which taken from health children (1 sample each person) were chosen as control group(n=35). The expression of T lymphocyte subsets CD3+, CD3+CD4+, CD3+CD8+, CD4+CD25+ Treg and Foxp3 mRNA were compared among three groups. There was no significant difference in age and sex distribution between IM patients and health chirdren (P>0.05). The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Sichuan Province People's Hospital.Informed consent was obtained from each parents.

Results

There had significant differences among three groups in relative expression levels of CD3+, CD3+CD4+, CD3+CD8+, CD4+CD25+ Treg (P<0.01). The relative levels of Foxp3 mRNA in IM acute stage group(2.82±0.90) were obviously lower than those of IM recovery stage group(4.11±1.37) and control group (4.65±1.23) (P<0.01). There was no significant difference between IM recovery stage group and control group(P>0.05). A significant positive correlation was found between CD4+CD25+ Treg and expression of Foxp3 mRNA in IM acute stage group(r=0.823, P<0.05).

Conclusions

There is functional disorder of T lymphocyte subsets at acute stage of IM in children. The relative expression levels of CD4+CD25+ Treg were reduced, and their special transcription factor Foxp3 mRNA were decreased, which led to the insufficient immunosuppressive effects that may be one of the important reasons of the immune imbalance.

表1 3组CD3+,CD3+ CD4+,CD3+CD8+,CD4+/CD8+的相对表达水平比较(±s)
Table 1 Comparison of the relative expression levels of CD3+,CD3+CD4+,CD3+CD8+,CD4+/CD8+ among three groups(±s)
表2 3组Foxp3 mRNA,CD4+CD25+ Treg相对表达水平比较(±s)
Table 2 Comparison of the relative expression levels of Foxp3 mRNA, CD4+CD25+ Treg among three groups(±s)
图1 CD4+CD25+Treg与Foxp3 mRNA的相关性分析图
Figure 1 The scatter of CD4+CD25+Treg and Foxp3 mRNA
[1]
Michelow P, Wright C, Pantanowitz LA.Review of the cytomorphology of epstein-barr virus-associated malignancies[J].Acta Cytol, 2012, 56(1):1-14.
[2]
Okano M, Gross TG.Acute or chronic life-threatening diseases associated with Epstein-Barr virus infection[J].Am J Med Sci, 2011, 343(6):1-7.
[3]
Ambinder RF. Epstein-Barr virus and Hodgkin lymphoma[J]. Hematol Am Soc Hematol Educ Program, 2007:204-209.
[4]
Mueller NE, Lennette ET, Dupnik K, et al. Antibody titers against EBNA1 and EBNA2 in relation to Hodgkin lymphoma and history of infectious mononucleosis[J]. Int J Cancer, 2012, 130(12):2886-2891.
[5]
Sakaguchi S, Sakaguchi N, Asano M, et al. Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases[J]. J Immunol, 1995, 155(3):1151-1164.
[6]
Xie ZD. Clinical features and diagnositc criteria of infectious mononucleosis associated with Epstein-Barr virus infection in children[J]. J Appl Clin Pediatr, 2007, 22(22):1759-1760.
[7]
Piccirillo CA.Regulatory T cells in health anddisease[J].Cytokine, 2008, 43(3):395-401.
[8]
Wingate PJ, McAulay KA, Anthony IC, et al. Regulatory T cell activity in primary and persistent Epstein-Barr virus infection[J]. J Med Virol, 2009, 81:870-877.
[9]
Wilczynki JR, Radwan M, Kalinka J.The characterization and role of regulatory T cells in immune reactions[J].Front Biosci, 2008, 1(13):2266-2227.
[10]
Sharma R, Zheng L, Deshmukh US, et al. A Regulatory T Cell-dependent novel function of CD25+ controlling memory CD8+ T cell homeostasis[J].J Immunol, 2007, 178(3):1251-1125.
[11]
Cao X, Cai SF, Fehniger TA, et al. Granzyme B and perforin are important for regulatory T cell-mediated suppression of tumor clearance[J]. Immunity, 2007, 279(4):635-646.
[12]
Pandiyan P, Zheng L, Ishihara S, et al.CD4+CD25+Foxp3 regulatory T cells induce cytokine deprivation-mediated apoptosis of effector CD4+ T cells[J]. Nat Immunol, 2007:1353-1362.
[13]
Sakaguchi S, Yamaguchi T, Nomura T, et al. Regulatory T cells and immune tolerance[J]. Cell, 2008, 133(5):775-787.
[14]
Chow YH, Chang HW, Sia R, et al.Tonsillar CD4+Foxp3 T-regulatory cell dynamics in primary EBV infection[J].Immunol Res, 2011, 50(1):97-101.
[15]
Ziegler SF. Foxp3 of mice and men[J].Annu Rev Immunol, 2006, 24:209-226.
[16]
Fontenot JD, Rasmussen JP, Williams LM, et al.Regulatory T cell lineage specification by the forkhead transcription factor Foxp3[J]. Immunity, 2005, 22(3):329-341.
[17]
Wang Q, Dong W, Fang J, et al. Roles of CD4+CD25+ T regulatory cells in acute infectious mononucleosis in children[J].J Appl Clin Pediatr, 2009, 24(22):1722-1728.
[18]
Qiu LN, Zhou YL, Luo XM, et al.Study of the inhibition effects of CD4+CD25+ /highCD127low on the immortalized cells in children with infectious mononucleosis[J]. Chin J Microbiol Immunol, 2009, 29(7):623-626.
[19]
Cao YD, Nie SD, Deng ZH, et al. Dynamic analysis of T lymphocyte subsets in children with infectious mononucleosis[J]. Int J Lab Med, 2012, 33(1):20-22.
[20]
Margaret F, Callan C. The evolution of antigen-specific CD8 T cell responses after matural primary infection of humans with Epstein-Barr virus[J].Viral Immunol, 2003, 6(1):3-16.
[21]
Precopio ML, Sullivan JL, Willard C, et al. Differential kinetics and specificity of EBV-specific CD4+ and CD8+ T cells during primary infection[J].J Immunol, 2003, 170(5):2590-2598.
[22]
Ren SH, Wang Q, Fang J, et al. Dynamic changes of T lymphocyte subset and B cells in children with infectious mononucleosis caused by epstein-barr virus[J/CD].Chin J Obstet Gynecol Pediatr:Electron Ed, 2009, 5(5):7-1.
[1] 陶宏宇, 叶菁菁, 俞劲, 杨秀珍, 钱晶晶, 徐彬, 徐玮泽, 舒强. 右心声学造影在儿童右向左分流相关疾病中的评估价值[J/OL]. 中华医学超声杂志(电子版), 2024, 21(10): 959-965.
[2] 刘琴, 刘瀚旻, 谢亮. 基质金属蛋白酶在儿童哮喘发生机制中作用的研究现状[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(05): 564-568.
[3] 向韵, 卢游, 杨凡. 全氟及多氟烷基化合物暴露与儿童肥胖症相关性研究现状[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(05): 569-574.
[4] 张梦思, 麻艺群, 蒙礼娟, 朱辉, 付晋凤. 压力手套与指蹼加压带及泡沫型硅凝胶贴膜联合应用于儿童瘢痕性并指术后的效果观察[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(04): 329-334.
[5] 郑宝英, 黄小兰, 贾楠, 朱春梅. 儿童难治性肺炎支原体肺炎早期预警指标[J/OL]. 中华实验和临床感染病杂志(电子版), 2024, 18(04): 215-221.
[6] 刘冉佳, 崔向丽, 周效竹, 曲伟, 朱志军. 儿童肝移植受者健康相关生存质量评价的荟萃分析[J/OL]. 中华移植杂志(电子版), 2024, 18(05): 302-309.
[7] 丁荷蓓, 王珣, 陈为国. 七氟烷吸入麻醉与异丙酚静脉麻醉在儿童腹股沟斜疝手术中的应用比较[J/OL]. 中华疝和腹壁外科杂志(电子版), 2024, 18(05): 570-574.
[8] 薛春娥, 赵娟娟, 刘韦成, 张义亮, 马毓晗, 李迎巧. CD4+CD25+foxp3+调节性T细胞通过NKG2D增强急性髓性白血病中NK细胞毒性[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(04): 212-219.
[9] 中华医学会器官移植学分会, 中华医学会外科学分会外科手术学学组, 中华医学会外科学分会移植学组, 华南劈离式肝移植联盟. 劈离式供肝儿童肝移植中国临床操作指南[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 593-601.
[10] 刘军, 丘文静, 孙方昊, 李松盈, 易述红, 傅斌生, 杨扬, 罗慧. 在体与离体劈离式肝移植在儿童肝移植中的应用比较[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 688-693.
[11] 张佳臣, 宋红欣. 儿童青少年等效球镜屈光度变化与屈光不正进展相关性的临床研究[J/OL]. 中华眼科医学杂志(电子版), 2024, 14(04): 217-222.
[12] 张琛, 秦鸣, 董娟, 陈玉龙. 超声检查对儿童肠扭转缺血性改变的诊断价值[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(06): 565-568.
[13] 王晓瑜, 郭群英, 牛雅萌, 赵成松. 公立儿童医院促进儿科就医均等化实践探析[J/OL]. 中华临床医师杂志(电子版), 2024, 18(04): 383-387.
[14] 陈晓胜, 何佳, 刘方, 吴蕊, 杨海涛, 樊晓寒. 直立倾斜试验诱发31 秒心脏停搏的植入心脏起搏器儿童一例并文献复习[J/OL]. 中华脑血管病杂志(电子版), 2024, 18(05): 488-494.
[15] 曹亚丽, 高雨萌, 张英谦, 李博, 杜军保, 金红芳. 儿童坐位不耐受的临床进展[J/OL]. 中华脑血管病杂志(电子版), 2024, 18(05): 510-515.
阅读次数
全文


摘要