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中华妇幼临床医学杂志(电子版) ›› 2011, Vol. 07 ›› Issue (03) : 202 -205. doi: 10.3877/cma.j.issn.1673-5250.2011.03.004

论著

TCT标本检测高危HPV E6/E7mRNA及在宫颈病变中的应用研究
刘桐宇, 谢榕, Lulu Zhang, 郑雄伟, 陈刚, 何诚, 林玉珍, 杨琳, 李桑   
  1. 350014 福建福州,福建省肿瘤医院妇科
    美国纽约罗切斯特大学肿瘤放射中心
    350014 福建福州,福建省肿瘤医院病理科
  • 出版日期:2011-06-01

Practicability Study of Applying High-Risk Human Papilloma Virus E6/E7 mRNA Levels to Determine Cervical Lesion by Using ThinPrep Cytology Test Sample

Tong-yu LIU, Rong XIE, Lulu Zhang, Xiong-wei ZHENG, Gang CHEN, Cheng HE, Yu-zhen LIN, Lin YANG, Sang LI   

  1. Department of Gynecology, Fujian Tumor Hospital, Fuzhou 350014, Fujian Province, China
  • Published:2011-06-01
  • Supported by:
    * Project No. 2009-2-29, supported by the Fund for Young Scholars From Shangdong Provincial Health Department
引用本文:

刘桐宇, 谢榕, Lulu Zhang, 郑雄伟, 陈刚, 何诚, 林玉珍, 杨琳, 李桑. TCT标本检测高危HPV E6/E7mRNA及在宫颈病变中的应用研究[J]. 中华妇幼临床医学杂志(电子版), 2011, 07(03): 202-205.

Tong-yu LIU, Rong XIE, Lulu Zhang, Xiong-wei ZHENG, Gang CHEN, Cheng HE, Yu-zhen LIN, Lin YANG, Sang LI. Practicability Study of Applying High-Risk Human Papilloma Virus E6/E7 mRNA Levels to Determine Cervical Lesion by Using ThinPrep Cytology Test Sample[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2011, 07(03): 202-205.

目的

探讨新柏超薄液基细胞学检测(Thin-prep cytology test, TCT)标本检测高危人乳头瘤病毒(human papilloma virus, HPV) E6/E7 mRNA可行性及在宫颈癌筛查中的应用价值。

方法

两种标本处理方法,分别在22例经超薄液基细胞学技术标本检测HPV E6/E7 mRNA及HPV E6/E7 DNA;对108例不同病变程度宫颈癌标本采取超薄液基细胞学技术检测高危HPV (high-risk HPV, HR-HPV) E6/ E7 mRNA及高危HPV E6/E7 DNA,并行统计学处理。

结果

两种方法处理标本行Willcoxon配对秩和检验比较,差异无显著意义(P>0.05)。随着宫颈病变程度增加,HPV E6/E7 mRNA表达率相应增加。新柏液基细胞学技术检测108例标本高危HPV E6/E7 mRNA表达的相对拷贝数为320.554±779.104,高危HPV E6/E7 DNA为1540.963±3575.255,两者秩相关检验,相关系数为1。

结论

超薄液基细胞学检测标本可行高危HPV E6/E7 mRNA及HPV E6/E7 DNA检测;宫颈癌筛查检测HPV E6/E7 mRNA有一定临床意义。

Objective

To research the feasibility and clinical application value of high-risk human papilloma virus (HR-HPV) E6/E7 mRNA and DNA transcripts in cervical cancer samples by using liquid-based Thin-prep cytology test (TCT).

Methods

Both high-risk HPV E6/E7 mRNA and DNA of 22 samples by two different methods of Specimen handling were detected. The data from detection of high-risk HPV E6/E7 mRNA and HPV E6/E7 DNA in 108 TCT samples with different cervical lesions were statistically analyzed.

Results

Willcoxon rank test was used to exam two different methods of Specimen handling (P> 0.05). The expression level of HPV E6/E7 mRNA transcripts was related to the development of severe epithelial cervical dysplasia. The expression copy number of HPV E6/E7 mRNA transcripts in 108 Thin-prep cytology test was 320.554 ± 779.104. The expression copy number of HPV E6/E7 DNA transcripts in 108 Thin-prep cytology tests was 1540.963 ± 3575.255. The coefficient of Spearman test among HPV DNA and E6/E7 mRNA was 1.

Conclusions

The residual Thin-prep cytology test samples could be used in the detection of high-risk HPV E6/E7 mRNA and HPV E6/E7 DNA. HPV E6 and E7 mRNA test could be a useful tool for cervical cancer screening.

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