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中华妇幼临床医学杂志(电子版) ›› 2011, Vol. 07 ›› Issue (02) : 101 -104. doi: 10.3877/cma.j.issn.1673-5250.2011.02.003

论著

妊娠期肝内胆汁淤积症患者胎盘组织中有机阴离子转运多肽-A,-C表达的研究
刘玉芳, 门晓亮, 崔陶, 吴琳, 邢爱耘   
  1. 610041 四川成都,四川大学华西第二医院妇产科
  • 出版日期:2011-04-01

Placental Expression of Organic Anion Transporting Polypeptide -A and -C in Intrahepatic Cholestasis of Pregnancy

Yu-fang LIU, Xiao-liang MEN, Tao CUI, Ling WU, Ai-yun XING   

  1. Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Published:2011-04-01
  • Supported by:
    * Project No. 30571964, supported by National Natural Foundation of China
引用本文:

刘玉芳, 门晓亮, 崔陶, 吴琳, 邢爱耘. 妊娠期肝内胆汁淤积症患者胎盘组织中有机阴离子转运多肽-A,-C表达的研究[J]. 中华妇幼临床医学杂志(电子版), 2011, 07(02): 101-104.

Yu-fang LIU, Xiao-liang MEN, Tao CUI, Ling WU, Ai-yun XING. Placental Expression of Organic Anion Transporting Polypeptide -A and -C in Intrahepatic Cholestasis of Pregnancy[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2011, 07(02): 101-104.

目的

探讨有机阴离子转运多肽(organic anion transporting polypeptide, OATP)-A,-C在妊娠各期和妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)患者胎盘的表达。

方法

收集2006年5月至2007年2月,在本院产科确诊为肝内胆汁淤积患者纳入ICP组(n=20);同期在门诊手术室行终止妊娠术的正常早孕期妇女8例,在计划生育科采用米非司酮+米索前列醇或水囊引产的正常中孕期妇女7例及在产科手术室行剖宫产术正常分娩的晚孕期妇女20例,纳入对照组(n=35)(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象本人的知情同意,并与之签署临床研究知情同意书)。两组胎盘组织中OATP-A mRNA,OATP-C mRNA测定,采用实时荧光定量PCR法;胎盘组织中有机阴离子转运多肽-A细胞学定位,采用免疫组织化学法。

结果

①对照组妊娠各期及ICP组胎盘组织中均检出OATP-A mRNA表达,均未发现OATP-C mRNA表达。对照组早、中、晚孕期胎盘组织中,OATP-A mRNA相对量比较,差异无显著意义(P>0.05);ICP组胎盘组织中,OATP-A mRNA相对量高于对照组(40.72±31.46 vs. 21.54±15.65),差异有显著意义(P<0.05)。②有机阴离子转运多肽-A在对照组晚孕期和ICP组胎盘的表达部位,均为滋养细胞,而在血管内皮细胞无表达。

结论

胆盐载体OATP-A mRNA在妊娠期肝内胆汁淤积症患者的胎盘组织表达增高,可能是机体对抗胆汁淤积的一种反应。

Objective

To investigate placental expression of cholate transporter organic anion transporting polypeptide (OATP)-A and -C during normal pregnancy and patient with intrahepatic cholestasis of pregnancy (ICP).

Methods

From May 2006 to February 2007, placentas were collected from 20 pregnancy complicated with intrahepatic cholestasis (ICP group). Meanwhile, 20 women termination pregnancy as well as plan centas from 8 1st trimester and 7 2nd trimester pregnancy were selected into control group (n=35). RT-PCR was applied for the measurement of placental OATP-A mRNA, OATP-C mRNA expression. This localization of OATP-A was analyzed by immunohistochemistry. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of West China Second University Hospital, Sichuan University. Informed consent was obtained from all participants.

Results

①OATP-A mRNA was detected in placentas in control group of 1st, 2 nd, 3 rd trimester and ICP group placentas, transcript of organic anion transporting polypeptide-C was not detected in these placentas. Placental OATP-A mRNA expression was higher in ICP group than that in control group (40.72±31.46 vs. 21.54±15.65, P<0.05). No significant difference was found in placental OATP-A mRNA expression among 1st, 2nd and 3rd trimester placentas in control group. ②organic anion transporting polypeptide-A expression was detected in trophocytes by immunohistochemistry.

Conclusion

OATP-A mRNA expression is up-regulated in intrahepatic cholestasis of pregnancy placenta, which may represent a compensatory (anti-cholestatic) mechanism for increased bile acids levels in intrahepatic cholestasis of pregnancy.

图1 对照组晚孕期胎盘组织SP法染色 (×400)
图2 ICP组胎盘组织SP法染色 (×400)
1 Glantz A,Marschall HU,Mattsson LA. Intrahepatic cholestasis of pregnancy: Relationships between bile acid levels and fetal complication rates[J]. Hepatology, 2004,40(2):467-474.
2 Setchell KD, Dumaswala R, Colombo C, et al. Hepatic bile acid metabolism during early development revealed from the analysis of human fetal gallbladder bile [J]. J Biol Chem, 1988, 263(32): 16637-16644.
3 Tauner M,Boyer JL. Bile salt transporters: Molecular characterization, function, and regulation [J]. Physiol Rev, 2003, 83: 633-671.
4 Patel P,Weerasekera N, Hitchins M,et al. Semi quantitative expression analysis of MDR3, FIC1, BSEP, OATP-A, OATP-C,OATP-D, OATP-E and NTCP gene transcripts in 1st and 3 rd trimester human placenta[J]. Placenta, 2003,24(1): 39-44.
5 Livak KJ,Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-DELTADELTACT Method[J]. Methods, 2001, 25(4): 402-408.
6 Macias RI,Pascual MJ,Bravo A,et al. Effect of maternal cholestasis on bile acid transfer across the rat placenta-maternal liver tandem [J]. Hepatology, 2000,31(4): 975-983.
7 Marin JJ,Bravo P,El-Mir MY,et al. ATP-dependent bile acid transport across microvillous membrane of human term trophoblast[J]. Am J Physiol, 1995,268: 685-694.
8 Young AM, Allen CE,Audus KL. Efflux transporter of the human placenta[J]. Adv Drug Deliv Rev, 2003,55(1): 125-132.
9 Kullak-Ublick GA,Ismair MG,Stieger B,et al. Organic anion-transporting polypeptide B (OATP-B) and its functional comparison with three other OATPs of human liver [J]. Gastroenterology, 2001,120(2):525-533.
10 St-Pierre MV,Kullak-Ublick GA,Hagenbuch B,et al. Transport of bile acids in hepatic and non-hepatic tissues [J]. J Exp Biol, 2001,204(Pt 10): 1673-1686.
11 Briz O,Serrano MA,MacIas RI,et al. Role of organic anion-transporting polypeptides, OATP-A, OATP-C and OATP-8, in the human placenta-maternal liver tandem excretory pathway for foetal bilirubin [J]. Biochem J, 2003, 371(Pt 3): 897-905.
12 Ugele B, St-Pierre MV, Pihusch M, et al. Characterization and identification of steroid sulfate transporters of human placenta [J]. Am J Physiol Endocrinol Metab, 2003,284(2): E390-398.
13 Serrano MA,Brites D,Larena MG,et al. Beneficial effect of ursodeoxycholic acid on alterations induced by cholestasis of pregnancy in bile acid transport across the human placenta [J]. J Hepatol, 1998,28(5): 829- 839.
14 Serrano MA,Macias RI,Vallejo M,et al. Effect of ursodeoxycholic acid on the impairment induced by maternal cholestasis in the rat placenta-maternal liver tandem excretory pathway [J]. J Pharmacol Exp Ther, 2003, 305(2): 515-524.
15 Hagenbuch B,Meier PJ.The superfamily of organic anion transporting polypeptides[J]. Biochim Biophys Acta, 2003, 1609(1):1-18.
16 Ke JC, Xu Z, Xing AY, et al. Study on the placental expression of MDR3 in intrahepatic cholestasis of pregnancy [J]. Chin J Perinat Med, 2006, 9(4): 236-239. [柯柬初,徐洲,邢爱耘,等. 胎盘MDR3的表达及其与妊娠期肝内胆汁淤积症关系的研究[J].中华围产医学杂志,2006, 9(4): 236-239.]
17 Xu Z,Ke JC, Xing AY, et al. Study on relationship between expression of familial intrahepatic cholestasis 1 mRNA in placenta and intrahepatic cholestasis of pregnancy[J]. J Sichuan Uni (Med Sci Ed), 2008, 3: 430-433. [徐洲,柯柬初,邢爱耘.胎盘FIC1 mRNA的表达及其与ICP关系的研究[J].四川大学学报:医学版,2008, 3: 430-433.]
18 Cui T, Men XL, Liu YF, et al. Study on the placental expression of MRP in intrahepatic cholestasis of pregnancy [J]. J Sichuan Uni (Med Sci Ed), 2008,6: 1043-1045. [崔陶,门晓亮,刘玉芳,等. 胎盘胆盐载体MRP的表达及其与妊娠期肝内胆汁淤积症的关系[J]. 四川大学学报:医学版,2008,6: 1043-1045.]
19 Trauner M,Wagner M,Fickert P,et al. Molecular regulation of hepatobiliary transport systems: Clinical implications for understanding and treating cholestasis[J]. J Clin Gastroenterol, 2005, 39(4 Suppl 2):S111-124.
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