选择人巨细胞病毒(human cytomegalovirus, HCMV)基因序列设计分子信标，建立扩增体系及分子信标(molecular beacon)芯片检测方法。

选取2008年6月1日至2008年7月30日，人巨细胞病毒呈阳性的临床血标本来源于重庆市四所医院(重庆市儿童医院为9例、重庆市计生医院为8例、第三军医大学西南医院为7例、第三军医大学新桥医院为5例)，共计29例纳入感染组。选取同期来源于上述四所医院(第三军医大学西南医院为5例、重庆市计生医院为4例、第三军医大学新桥医院为4例、重庆市儿童医院为3例)的人巨细胞病毒呈阴性临床标本，共计16例纳入对照组。无探针包被位点(n＝10)纳入空白对照组(本研究遵循的程序符合第三军医大学西南医院人体试验委员会所制定的伦理学标准，得到该委员会批准，取得受试对象的知情同意，并与试验患儿监护人签署临床研究知情同意书)。运用Beacon designer软件设计人巨细胞病毒分子信标及其单侧延长臂分子信标，建立扩增体系及分子信标芯片人巨细胞病毒检测方法，应用荧光显微镜观测荧光信号强度。

荧光显微镜观测人巨细胞病毒感染组与对照组PCR产物与分子信标杂交后荧光信号强度区别明显；29例人巨细胞病毒婴幼儿感染(感染组)与16例无人巨细胞病毒感染(对照组)的荧光信号强度比较，差异有显著意义(P<0.05)。

分子信标芯片技术检测婴幼儿人巨细胞病毒基因具有灵敏、快速、可批量处理等优特点。采用荧光显微镜观测荧光杂交信号具有更强的荧光信号识别、放大功能及结果判断更准确等优点。

To design molecular beacon of human cytomegalovirus (HCMV) gene and its amplification system and find the detecting way by molecular beacon chip.

From June 1, 2008 to July 30, 2008, clinical positive human cytomegalovirus blood samples were collected from the following 4 hospitals, Southwest Hospital, Third Military Medical University (n=7), Chongqing Family Planning Hospital (n=8), Xinqiao Hospital of the Third Military Medical University(n=5), and Children's Hospital of Chongqing (n=9). A total of 29 cases were involved into infected group. At the same time, the clinical negative samples were collected from the same 4 hospitals, including Southwest Hospital, Third Military Medical University (n=5), Chongqing Family Planning Hospital (n=4), Xinqiao Hospital of the Third Military Medical University (n=4) and Children's Hospital of Chongqing(n=3). There were 16 cases were classified into the control. The points without probe (n=10) were designed in blank control group. The research procedures were fitted for the ethical standard formulated by the investigation in human beings committee of Southwest Hospital, Third Military Medical University. Informed consent was obtained from all participations. The software of Beacon designer was used to design the molecular beacon and molecular beacon with single extending arm of human cytomegalovirus gene. The amplification system and molecular beacon chip, and Fluorescence microscope and image analysis software were used to detect the fluorescent light and make a qualitative judgment.

The difference between PCR products from infected group and control group was obvious in detecting the fluorescent light by the Fluorescence microscope. The fluorescent light was obviously different between 29 cases from infected group and 16 cases from control group (P<0.05).

The technology of molecular beacon chip is a technology with characteristics of high sensitivity, quick and batch in detecting infant human cytomegalovirus gene. It has many advantages such as stronger fluorescent light recognition and amplification and more accurate in signal detection.