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中华妇幼临床医学杂志(电子版) ›› 2008, Vol. 04 ›› Issue (05) : 426 -431. doi: 10.3877/cma.j.issn.1673-5250.2008.05.106

论著

酒泉地区婴幼儿腹泻的A组轮状病毒分子基因特征研究
陈缤, 霍燕凤, 金玉, 方肇寅   
  1. 甘肃省酒泉市人民医院儿科(甘肃酒泉,735000)
    兰州大学第一医院儿科
    中国疾病预防控制中心病毒病所腹泻室
  • 出版日期:2008-10-01

Genetic Profile of Rotavirus A Among the Acute Diarrhea Infants in Jiuquan Region

Bin CHEN, Yan-feng HUO, Yu JIN, Zhao-yin FANG   

  1. Department of the Pediatrics, People's Hospital of Jiuquan, Jiuquan 735000, China
  • Published:2008-10-01
引用本文:

陈缤, 霍燕凤, 金玉, 方肇寅. 酒泉地区婴幼儿腹泻的A组轮状病毒分子基因特征研究[J/OL]. 中华妇幼临床医学杂志(电子版), 2008, 04(05): 426-431.

Bin CHEN, Yan-feng HUO, Yu JIN, Zhao-yin FANG. Genetic Profile of Rotavirus A Among the Acute Diarrhea Infants in Jiuquan Region[J/OL]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2008, 04(05): 426-431.

目的

采用基因分型方法调查研究酒泉地区婴幼儿A组轮状病毒(rotavirus, RV)腹泻的基因分型特点。

方法

对2001年11月至2002年12月酒泉地区收集的腹泻婴幼儿324份粪便标本,电脑随机抽样选取204份采用酶联免疫吸附试验(enzyme-linked immunosorbant assay,ELISA)和逆转录-聚合酶链反应(RT-PCR),进行轮状病毒病原检测,扩增A组轮状病毒VP4和VP7基因,并对阳性标本进行基因分型。

结果

酶联免疫吸附试验法测得204份粪便标本的A组轮状病毒阳性率为54.9%(112/204)。对酶联免疫吸附试验呈阳性的标本进行逆转录-聚合酶链反应法检测A组轮状病毒G,P基因型,结果表明,酒泉地区流行的A组轮状病毒主要基因型为G3P[8]型(50.0%),其余依次为G1P[4](15.8%)、G3P[4](15.8%)、G1P[8](10.6%)、G2P[4](2.6%)和G1G3P[4](2.6%)、G1G3P[8](2.6%)。

结论

A组轮状病毒是酒泉地区婴幼儿非细菌性腹泻的主要病原,其中G3P[8]型是主要基因型。

Objective

To investigate genetic subtypes of rotavirus A prevailed among acute diarrhea infants in Jiuquan, China.

Methods

From November 2001 to December 2002, 204 stool specimens collected from both outpatients and inpatients diagnosed as acute infant diarrhea, were tested by enzyme-linked immunosorbant assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) to identify the rotavirus infection and subtypes respectively, at the Department of Pediatrics, the People's Hospital of Jiuquan. There were 14 gene loci in total of the two rotavirus genes, rotavirus A VP4 and VP7, tested in this research to uncover the major genotypes on the G and P subtypes.

Results

Enzyme-linked immunosorbant assay results showed that rotavirus A was present in 112 cases out of 204 specimens (54.9%) without gender priority. G type accounted for 82 cases from the ELISA positive samples, which had G1, G2, G9, G1G2 and G1G3 subgroups. Among randomly selected 53 G-positive genotype specimens, 38 cases were subtyped as P strain, including P[4] and P[8], and the remaining 15 cases lacked the marker for the P strain. The overlap analysis of G and P subtype for rotavirus A showed that G3P[8] was the dominant (50.0%) type, followed by G1P[4] (15.8%), G3P[4] (15.8%), G1P[8] (10.6%), G2P[4] (2.6%), G1G3P[4] (2.6%), G1G3P[8] (2.6%) sequentially.

Conclusion

Rotavirus A was the major pathological agent of the infant and young children diarrhea in Jiuquan, a region where the predominant rotavirus stain was found out to be G3P[8].

图1 A组RV阳性粪便标本G基因型RT-PCR扩增产物琼脂糖电泳图
图2 酒泉地区婴幼儿轮状病毒基因分型分布(NY:未分出型)
图3 A组RV阳性粪便标本P基因型巢式RT-PCR扩增产物琼脂糖电泳图
1 Parashar UD,Hummelman EG, Bresee JS,et al. Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis,2003,9(5):565-572.
2 Fang ZY, Jin SJ, Qin SM, et al. Serotypes of group A rotavirus isolates determined by PCR in Hebei and Henan provinces, China. Chin J Virol,1994,10:316-321.[方肇寅,晋圣瑾,秦树民等.PCR方法用于我国A组轮状病毒的分型研究.病毒学报,1994,10:316-321.]
3 Gouvea V,Ho MS,Glass RI,et al.Serotypes and electropherotypes of human rotavirus in the USA:1987-1989.J Infect Dis,1990,162:362-367.
4 Escona MD,Garmah GE,Annelise G,et al. Detection of an unusual human rotavirus train with G5P[8] specificity in a Cameroonian child with diarrhea. Clin Microbiol,2004,42(1):441-444.
5 Villena C,Gabrieli R, Pinto RM,et al.A large infantile gastroenteritis outbreak in Albania caused by multiple emerging rotavirus genotypes.Epidemiol Infect,2003,31(3):1105-1110.
6 Laird AR,Gentsch JR,Nakagomi T,et al.Characterization of serotype G9 rotavirus strains isolated in the United States and Indian from 1993 to 2001.J Clin Microbiol,2003,41(7):3100-3111.
7 Carl K,Nada BS,Enzo P,et al.Genetic and antigenic characterization of rotavirus sertype G9 strains isolated in Australia between 1997-2001. J Clin Microbiol,2003,41(8):3649-3654.
8 Zeng M,Zhu QY,Zhang Y,et al. Molecular epidemiologic survey of rotaviruses from infants and children with diarrhea in Shanghai. Chin J Pediatr,2004,42(1):10-15.[曾玫,朱启钅容,张又等.上海地区婴幼儿腹泻的轮状病毒分子流行病学研究.中华儿科杂志,2004,42(1):10-15.]
9 Fang ZY,Qi J,Yang H, et al. Serotype and genotype study of children rotaviruses isolated in 1998-1999 in China. Chin J Virol,2001,17(1):17-23.[方肇寅,齐锦,杨辉等.我国1998-1999年流行的婴幼儿腹泻轮状病毒的分型研究.病毒学报,2001,17(1):17-23.]
10 Fang ZY,Wang B,Kilgore PE,et al. Sentinel hospital surveillance fou rotavirus diarrhea in the People's Republic of China,August 2001-July 2003.J Infect Dis,2005,192:s94-s99.
11 Jin Y, Huang X, Fang ZY, et al. Molecular epidemiology of virus diarrhea among infants and young children in Lanzhou. Chin J Pract Pediatr, 2006,21(1):15-18.[金玉,黄湘,方肇寅等.兰州地区婴幼儿病毒性腹泻的分子流行病学研究.中国实用儿科杂志,2006,21(1):15-18.]
12 Santos N, Hoshino Y. Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol, 2005,15(1):29-56.
13 Zhang LJ, Du ZQ, Zhang Q, et al. Rotavirus surveillance data from Kunming Children's Hospital, 1998-2001. Chin J Epidemiol,2004,25(5): 396-399.[张丽杰,杜曾庆,章青等.昆明市儿童医院1998-2001年轮状病毒哨点监测分析.中华流行病学杂志,2004,25(5):396-399.]
14 Santos N, Hoshino Y. Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol, 2005,15(1):29-56.
15 O'Halloran F, Fanning S. Molecular genotyping of Irish rotavirus strains. Methods Mol Biol, 2004, 268: 89-102.
16 Iturriza-Gomara M, Kang G, Gray J. Rotavirus genotyping:Keeping up with an evolving population of human rotaviruses. J Clin Virol, 2004, 31(4):259-265.
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