探讨血管内皮生长因子(vascular endothelial growth factor，VEGF)反义寡核苷酸(antisense ODNs)对大鼠异位子宫内膜的抑制作用。

建立大鼠右侧壁腹膜子宫内膜异位症模型，4周后将建模成功的大鼠共计32只随机平均分为A，B，C，D 4组(每组8只)，腹腔内分别注射VEGF反义、正义、错义寡核苷酸2 000 μmol/100 μl Hank's液及生理盐水100 μl，隔日1次，共4周。治疗完成2周后处死大鼠，计算药物对异位内膜的抑制率，取卵巢、在位内膜及异位内膜进行组织学检查，并测定血清雌二醇(E₂)、孕酮(P)水平。

A组异位内膜体积显著缩小(P<0.05)，异位内膜抑制率显著高于其他3组(P<0.05);其他3组之间差异无显著意义(P>0. 05)。A组异位内膜腺体及腺上皮呈明显萎缩相，血清E₂，P水平与其他3组相比，有下降趋势，但差异无显著意义(P>0.05)。

腹腔注射VEGF反义寡核苷酸，可以抑制大鼠异位子宫内膜的生长，使异位内膜萎缩，而对卵巢功能无明显影响。

To investigate whether vascular endothelial growth factor (VEGF) antisense ODNs can prevent endometrial implantation in a rat model.

32 rats models of surgically induced endometriosis were averagely divided into 4 groups (A, B, C, D group). The rats were treated with2 000 μmol/100 μl VEGF antisense, sense, missense ODNs or saline 100 μl by injection into the abdomen respectively every 2 days for 4 weeks. the rats were killed at 2 weeks after the therapy was finished. The rate of transplantation inhibition was calculated by the formula (1 — V₂/V₁) × 100%. Vaginal cytology smears were examined everyday to determine the rats' estrus cycle, endometrium transplant, normal endometrium and ovary were resected for histological examination, and serum E₂, P level were detected by radioimmunoassay method.

Transplants volum of VEGF antisense ODNs treated rats (A group)was diminuted significantly compared with other 3 groups (P<0. 01), the rate of transplantation inhibition of VEGF antisense ODNs group (A group) was much higher than other 3 groups (P<0. 01). Transplants of VEGF antisense ODNs treated rats (A group) showed marked regression of both epithelial and stromal components, epithelium developed poorly, lamina propria was thinner with much fewer stromal cells and glands. The ovary function and the structure of the normal endometrium were not affected by VEGF antisense ODNs, nearly all rats showed normal estrus cycle, no significant difference of serum E₂, P level was found among 4 groups.

VEGF antisense ODNs could inhibit the growth and induce the regression of explants in a rat endometriosis model without affecting the ovary function.