成纤维细胞生长因子受体4在肾脏疾病患儿肾组织的表达与意义

doi:10.3877/cma.j.issn.1673-5250.2006.04.103

目的

明确成纤维细胞生长因子受体4(fibroblast growth factor receptor 4，FGFR 4)在肾脏病患儿肾组织的表达及肾脏病理发生中的作用。

方法

采用免疫组化法观察FGFR 4在原发性肾病综合征(28例)及急性肾小球肾炎(18例)、紫癜性肾炎(20例)，单纯性血尿(22例)患儿及正常肾组织(5例)的表达。分析FGFR 4表达与肾病理积分及临床指标的相关性。

结果

各临床及病理类型肾脏疾病足细胞、近端小管、远端小管FGFR 4的表达积分均明显高于正常组(P<0.05)；足细胞FGFR 4表达积分与肾小球病理积分、尿蛋白呈负相关；近端小管FGFR 4表达积分与肾小管病理积分、尿蛋白呈正相关；远端小管FGFR 4表达积分与肾小管病理积分、尿蛋白呈负相关。

结论

FGFR 4参与肾脏病理损害的发生。不同组织的FGFR 4作用不同：对足细胞和远端小管起保护作用，而对近端小管起损害作用。

关键词: 成纤维细胞生长因子受体4, 原发性肾病综合征, 急性肾小球肾炎, 紫癜性肾炎, 单纯性血尿

Objective

To investigate the expression and role of fibroblast growth factor receptor 4(FGFR 4)in renal tissues from the children with renal disease.

Methods

Paraffin-embedded renal biopsy tissue sections from 28 children with primary nephrotic syndrome(PNS), 18 children with acute glomerulonephritis(AGN), 20 children with Henoch-Schoenlein purpura nephritis(HSPN)and 22 children with isolated hematuria(IHU)were examined by immunohistochemical staining with anti- FGFR 4 monoclonal antibody.Normal renal tissues from 5 nephrectomized patients with nephroma were selected as control.Possible correlation between the expression of FGFR 4 and pathologic lesion score, clinical index of renal disease was analysed.

Results

The expression of FGFR 4 in proximal tubules, distal tubules and podocytes of children with renal disease are all significantly higher than that of the control group(P< 0.05).The expression of FGFR 4 in podocytes show negative correlation with pathologic lesion score of glomerulus and proteinuria(P<0.05).The expression of FGFR 4 in proximal tubules show positive correlation with pathologic lesion score of tubules and proteinuria(P<0.05).The expression of FGFR 4 in distal tubules show negative correlation with pathologic lesion score of tubules and proteinuria(P< 0.05).

Conclusions

The over expression of FGFR 4 take part in the onset and development of renal disease, but the role of FGFR 4 is different in different tissues: protective factor to podcytes and distal tubules, harmful factor to proximal tubules.

Key words: fibroblast growth factor receptor 4, primary nephrotic syndrome, acute glomerulonephritis, Henoch-Schoenlein purpura nephritis, isolated hematuria

图1　肾脏疾病肾组织FGFR4免疫组化染色结果(X400)。(→)为FGFR4微弱阳性足细胞，(△)为FGFR4强阳性近端小管上皮细胞，且见肾小管呈明显扩张、萎缩状态。

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Expression and role of fibroblast growth factor receptor 4 in renal tissues from the children with renal disease

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Expression and role of fibroblast growth factor receptor 4 in renal tissues from the children with renal disease