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中华妇幼临床医学杂志(电子版) ›› 2015, Vol. 11 ›› Issue (01) : 14 -17. doi: 10.3877/cma.j.issn.1673-5250.2015.01.004

所属专题: 文献

论著

叶黄素对人宫颈癌HeLa细胞增殖和凋亡的影响
刘会芳1, 赵元华1, 何荣霞1,*,*(), 王芳1   
  1. 1. 730030 兰州大学第二医院妇产科
  • 收稿日期:2014-08-09 修回日期:2014-12-29 出版日期:2015-02-01
  • 通信作者: 何荣霞

Effects of lutein on cell proliferation and apoptosis of human cervical cancer HeLa cells

Huifang Liu1, Yuanhua Zhao1, Rongxia He1(), Fang Wang1   

  1. 1. Department of Gynaecology and Obstetrics, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China
  • Received:2014-08-09 Revised:2014-12-29 Published:2015-02-01
  • Corresponding author: Rongxia He
  • About author:
    Corresponding author: He Rongxia, Email:
引用本文:

刘会芳, 赵元华, 何荣霞, 王芳. 叶黄素对人宫颈癌HeLa细胞增殖和凋亡的影响[J/OL]. 中华妇幼临床医学杂志(电子版), 2015, 11(01): 14-17.

Huifang Liu, Yuanhua Zhao, Rongxia He, Fang Wang. Effects of lutein on cell proliferation and apoptosis of human cervical cancer HeLa cells[J/OL]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2015, 11(01): 14-17.

目的

探讨叶黄素对体外培养的人宫颈癌HeLa细胞增殖和凋亡的影响。

方法

选择对数生长期的人宫颈癌HeLa细胞为研究对象,采用随机数字表法,将其随机分为6组:20,40,80,160,320 μmol/L叶黄素组及对照组,叶黄素组分别加入200 μL含20,40,80,160,320 μmol/L叶黄素终浓度的RPMI1640培养基及HeLa细胞,对照组仅加入200 μL RPMI1640培养基及HeLa细胞。采用噻唑蓝(MTT)法检测人宫颈癌HeLa细胞增殖抑制率,并采用流式细胞术检测HeLa细胞凋亡率。采用统计学方法分析相同时间不同浓度叶黄素培养及相同浓度叶黄素培养不同时间的HeLa细胞增殖抑制率及凋亡率差异。

结果

HeLa细胞在培养时间相同(为24 h或48 h或72 h)时,随着叶黄素浓度依次增加(分别为20,40,80,160,320 μmol/L)均较前一低浓度组的增殖抑制率及凋亡率显著升高,且差异均有统计学意义(P<0.01);HeLa细胞在叶黄素浓度相同(为20 μmol/L或40 μmol/L或80 μmol/L或160 μmol/L或320 μmol/L)培养时,随着培养时间增加(分别为24,48,72 h)均较前一时间点的增殖抑制率及凋亡率显著升高,且差异有统计学意义(P<0.01)。

结论

叶黄素可抑制体外培养的人宫颈癌HeLa细胞的增殖,诱导其凋亡,并且HeLa细胞体外培养的增殖抑制率及凋亡率分别随着叶黄素浓度增加及作用时间延长而升高。

Objective

To observe the effects of lutein on cell proliferation and apoptosis of in vitro culture human cervical cancer HeLa cells.

Methods

Select human HeLa cells of the logarithmic growth phase for the study. Using a random number table method, those HeLa cells were randomly divided into six groups: 20, 40, 80, 160, 320 μmol/L lutein group and control group, then added 200 μL RPMI1640 medium contained final concentration of 20, 40, 80, 160, 320 μmol/L lutein and HeLa cells in 20, 40, 80, 160, 320 μmol/L lutein group, respectively, and added 200 μL RPMI1640 medium only and HeLa cells in control group.The HeLa cells proliferation inhibitory rates were determined by methyl thiazolyl tetrazolium(MTT) assay and the apoptosis rates of HeLa cells were determined by flow cytometry.Analyzed rates of proliferation inhibitory and apoptosis of HeLa cells that cultured the same time but in different concentrations of lutein and cultured in the same concentration of lutein but at different time points by statistical method.

Results

When HeLa cells cultured the same time(24 h or 48 h or 72 h), with lutein concentration increased by turns(20, 40, 80, 160, 320 μmol/L, respectively), compared with the previous low concentration group, the proliferation inhibitory rates and apoptosis rates increased significantly, and the differences were statistically significant(P<0.01); When HeLa cells cultured in the same concentration(20 μmol/L or 40 μmol/L or 80 μmol/L or 160 μmol/L or 320 μmol/L) of lutein, with the culture time extended (24, 48, 72 h, respectively), compared with the previous time point, the proliferation inhibitory rates and apoptosis rates were significantly increased, and the differences were statistically significant(P<0.01).

Conclusions

Lutein can inhibit the cell proliferation and induce the apoptosis of in vitro culture human HeLa cells.The rates of proliferation inhibitory and apoptosis of in vitro culture human HeLa cells could increase with lutein concentration increasing and culture time prolonging.

表1 相同时间不同浓度叶黄素培养及相同浓度叶黄素培养不同时间HeLa细胞增殖抑制率比较(%,±s)
Table 1 Comparison of proliferation inhibitory rates of HeLa cells cultured within the same time but in different concentrations of lutein,and cultured in the same concentration of lutein but at different time points(%,±s)
表2 相同时间不同浓度叶黄素培养及相同浓度叶黄素培养不同时间HeLa细胞凋亡率比较(%,±s)
Table 2 Comparison of apoptosis rates of HeLa cells cultured within the same time but in different concentrations of lutein,and cultured in the same concentration of lutein but at different time points(%, ±s)
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