切换至 "中华医学电子期刊资源库"
高级检索
中华妇幼临床医学杂志(电子版)

中华妇幼临床医学杂志(电子版) ›› 2014, Vol. 10 ›› Issue (06) : 774 -778. doi: 10.3877/cma.j.issn.1673-5250.2014.06.017

所属专题： 文献；

论著

妊娠期高血压疾病患者母、胎转化生长因子-β1、血管紧张素Ⅱ及血管内皮生长因子表达水平分析
巨容1,*,*(), 胡旭红1, 徐若梅1, 郑毅1, 傅益永1   
  1. 1. 610091　成都市妇女儿童中心医院新生儿科
  • 收稿日期:2014-08-09 修回日期:2014-11-06 出版日期:2014-12-01
  • 通信作者: 巨容

Analysis of Transforming Growth Factor-β1,Angiotensin Ⅱ and Vascular Endothelial Growth Factor Expression Levels in Women With Hypertensive Disorder Complicating Pregnancy

Rong Ju1(), Xuhong Hu1, Ruomei Xu1, Yi Zheng1, Yiyong Fu1   

  1. 1. Department of Pediatrics, Chengdu Women's & Children's , CentraI Hospital, Chengdu 610091, Sichuan Province, China
  • Received:2014-08-09 Revised:2014-11-06 Published:2014-12-01
  • Corresponding author: Rong Ju
  • About author:
    (Corresponding author : Ju Rong,Email: )
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

巨容, 胡旭红, 徐若梅, 郑毅, 傅益永. 妊娠期高血压疾病患者母、胎转化生长因子-β1、血管紧张素Ⅱ及血管内皮生长因子表达水平分析[J]. 中华妇幼临床医学杂志(电子版), 2014, 10(06): 774-778.

Rong Ju, Xuhong Hu, Ruomei Xu, Yi Zheng, Yiyong Fu. Analysis of Transforming Growth Factor-β1,Angiotensin Ⅱ and Vascular Endothelial Growth Factor Expression Levels in Women With Hypertensive Disorder Complicating Pregnancy[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2014, 10(06): 774-778.

目的

比较妊娠期高血压疾病孕妇与正常孕妇外周血、脐带血和胎盘中转化生长因子(TGF)-β1、血管紧张素(Ang) Ⅱ和血管内皮生长因子(VEGF)的表达差异，为探讨妊娠期高血压疾病的发病机制提供临床依据。

方法

选择2009年1月至2012年12月在成都市妇女儿童中心医院分娩且合并妊娠期高血压疾病，但无其他妊娠合并症的50例孕妇为研究对象，纳入妊娠期高血压疾病组，选择同期在同一家医院分娩的50例正常孕妇纳入对照组。在孕妇分娩时留取胎盘母、子面胎盘组织，母亲静脉血及新生儿脐静脉血，采用酶联免疫吸附测定(ELISA)法测定TGF-β1、AngⅡ和VEGF的表达水平，并进行统计学分析。两组孕妇年龄、孕龄及体质量比较，差异均无统计学意义(P>0.05)。本研究遵循的程序符合成都市妇女儿童中心医院人体试验委员会制定的伦理学标准，得到该委员会批准，分组征得受试对象知情同意，并与之签署临床研究知情同意书。

结果

①妊娠期高血压疾病组母亲静脉血、新生儿脐静脉血、胎盘母面及胎盘子面组织中TGF-β1表达水平均较对照组低，而Ang Ⅱ表达水平均较对照组高，且差异均有统计学意义(P<0.05)；②妊娠期高血压疾病组新生儿脐静脉血、胎盘母面及胎盘子面组织中VEGF表达水平均较对照组低，且差异有统计学意义(P<0.05)；但两组母亲静脉血VEGF表达水平比较，差异无统计学意义(t=0.639, P>0.05)。

结论

TGF-β1、AngⅡ和VEGF表达水平的改变在妊娠期高血压疾病的发生、发展中有重要作用。

关键词: 高血压，妊娠性, 转化生长因子-β, 血管紧张素Ⅱ, 血管内皮生长因子
Objective

To compare the expression level differences of transforming growth factor (TGF)-β1, angiotensin (Ang) Ⅱ and vascular endothelial growth factor (VEGF) in maternal blood, umbilical cord blood and the placenta surface in women with hypertensive disorder complicating pregnancy and normal pregnant women, and provide the clinical evidence for exploring the mechanisms of hypertensive disorder complicating pregnancy.

Methods

A total of 50 pregnant women with hypertensive disorder complicating pregnancy without other complications who delivered in Chengdu Women's & Children's Central Hospital from January 2009 to December 2012 were bringing into study group and 50 normal pregnant women who delivered at the same hospital and same time were bringing into control group. Placenta tissue of maternal and fetal surface,maternal blood and umbilical cord blood during childbirth were obtained. The expression levels of TGF-β1, Ang Ⅱ and VEGF were detected by enzyme linked immunosorbent assay (ELISA) and those expression levels were analyzed statistically. There were no statistically significant differences between two groups in maternal age,gestational age and body weight(P> 0.05). This study followed the Ethics Standards of Human Trial Committee of Chengdu Women's & Children's Central Hospital, and was approved by the Human Trial Committee. All subjects signed the informed consent.

Results

①The expression levels of TGF-β1 in maternal blood, umbilical cord blood, placenta maternal surface and fetal surface in hypertensive disorder complicating pregnancy group were significantly lower than those in control group while the expression levels of Ang Ⅱ were significantly higher,and the differences were statistically significant (P<0.05).②The expression levels of VEGF in umbilical cord blood, placenta maternal surface and fetal surface in hypertensive disorder complicating pregnancy group were significantly lower than those in control group, and the differences were statistically significant (P<0.05) ; But, there were no statistically significant differences between two groups of VEGF expression level in maternal blood (t = 0.639, P>0.05).

Conclusions

The change of expression levels of TGF-β1,Ang Ⅱ and VEGF plays an important role in occurrence and progress of hypertensive disorder complicating pregnancy.

Key words: Hypertension, pregnancy-induced, Transforming growth factor beta, Angiotensin Ⅱ, Vascular endothelial growth factor
表1 两组TGF-β1表达水平比较（ng/mL，±s）
Table 1 Comparison of TGF-β1 expression levels between two groups(ng/mL, ±s)
组别 例数 胎盘母面组织 胎盘子面组织 母亲静脉血 新生儿脐静脉血
妊娠期高血压疾病组 50 5.6±1.4 5.2±1.6 6. 0±1.8 6. 6±1.6
对照组 50 6.4±1.8 6. 0±1.6 6. 8±1.9 6.9±1.8
t值 2.707 2.525 2.032 2.099
P值 0.008 0.013 0.045 0.038
表2 两组AngⅡ表达水平比较（ng/mL，±s）
Table 2 Comparison of Ang Ⅱ expression levels between two groups(ng/mL,±s)
组别 例数 胎盘母面组织 胎盘子面组织 母亲静脉血 新生儿脐静脉血
妊娠期高血压疾病组 50 9.4±3.7 14.9±4.5 44.5±9.9 57.5±13.8
对照组 50 8.0±3.3 12.7±3.6 39.8±9.1 52.1±13.5
t值 2.014 2.651 2.495 2.004
P值 0.047 0.009 0.014 0.048
图1 妊娠期高血压疾病及正常孕妇胎盘母面VEGF表达水平比较（IHC染色，DAB显色，× 100）
Figure 1 Comparison of VEGF expression levels on maternal surface between women with hypertensive disorder complicating pregnancy and normal pregnant women(IHC staining,DAB coloration,× 100)
图2 妊娠期高血压疾病及正常孕妇胎盘子面VEGF表达水平比较（IHC染色，DAB显色，×100）
Figure 2 Comparison of VEGF expression levels on fetal surface of placenta between women with hypertensive disorder complicating pregnancy and normal pregnant women（IHC staining,DAB coloration,× 100）
表3 两组VEGF表达水平比较（ng/mL，±s）
Table 3 Comparison of VEGF expression levels between two groups(ng/mL, ±s)
组别 例数 胎盘母面组织 胎盘子面组织 母亲静脉血 新生儿脐静脉血
妊娠期高血压疾病组 50 19.7±8.7 19.6±6.8 20.5±7.9 18.2±5.3
对照组 50 23.7±11.1 26.1±9.6 21.7±8.1 23.6±8.7
t值 2.009 2.906 0.639 3.454
P值 0.047 0.000 0.524 0.001
[1]
蔡秋荣.妊娠期高血压疾病临床特点分析[J].中国妇幼保健，2012, 27(25):3910–3911.
[2]
Roelen BA, Goumans MJ, Zwijsen A, et al. Identification of two distinct functions for TGF-beta in early mouse development [J]. Differentiation, 1998, 64 (1) : 19–31.
[3]
Nielsen AH, Schauser KH, Poulsen K. Current topic: the uteroplacental renin-angiotensin system [J]. Placenta, 2000, 21 (5-6)：468–477.
[4]
李妹燕.血管内皮生长因子与妊娠及流产的研究进展[J].右江民族医学院学报，2008，30(5) :842–843.
[5]
Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF-mediated angiogenesis[J]. J Clin Invest, 2001, 107 (4)：409–417.
[6]
李芹，仇姝，王建军.VEGF蛋白在子痫前期胎盘中的表达及意义[J].河北医学，2010，16(10): 1157–1160.
[7]
耿丽红，蔡霞，王冬梅，等.妊高征胎盘中VEGF的表达和滋养细胞分泌功能的关系[J].新疆医科大学学报，2003，26(5)：467–469.
[8]
Svedas E, Islam KB, Nisell H, et al. Vascular endothelial growth factor induced functional and morphologic signs of endothelial dysfunction in isolated arteries from normal pregnant women[J]. Am J Obstet Gynecol, 2003, 188(1) : 168–176.
[9]
Jaeger LA, Spiegel AK, Ing NH, et al. Functional effects of transforming growth factor beta on adhesive properties of porcine trophectoderm[J]. Endocrinology, 2005, 146(9) : 3933–3942.
[10]
Li RH, Zhuang LZ. The effects of growth factors on human normal placental cytotrophoblast cell proliferation[J]. Hum Reprod，1997，12(4)：830–834.
[11]
Cox BE, Word RA, Rosenfeld CR. Angiotensin Ⅱ receptor characteristics and subtype expression in uterine arteries and myometrium during pregnancy[J]. J Clin Endocrinol Metab, 1996, 81(1)：49–58.
[12]
McMullen JR, Gibson KJ, Lumbers ER, et al. Interactions between AT1 and AT2 receptors in uterine arteries from pregnant ewes [J]. Eur J Pharmacol, 1999, 378(2) : 195–202.
[13]
Poston L. The control of blood flow to the placenta [J]. Exp Physiol, 1997, 82(2):377–387.
[14]
Phillips MI, Kagiyama S. Angiotensin Ⅱ as a pro-inflammatory mediator[J]. Curr Opin Investig Drugs，2002，3(4) : 569–577.
[1] 王博, 白子锐, 李坚. 近红外二区新型血管内皮生长因子受体靶向探针在结直肠癌小鼠模型中的应用[J]. 中华普通外科学文献(电子版), 2023, 17(03): 173-177.
[2] 芦丹, 杨硕, 刘旭. VEGF、HMGB1、hs-CRP/Alb在AECOPD伴呼吸衰竭中的变化及预后分析[J]. 中华肺部疾病杂志(电子版), 2023, 16(04): 532-534.
[3] 王玉芹, 刘冠群, 曲福君. IDH1和VEGF对非小细胞肺癌的诊断意义[J]. 中华肺部疾病杂志(电子版), 2022, 15(06): 847-849.
[4] 何继强, 任安伟, 王飞. 周围型肺癌CT特征与PCNA、VEGF及Bcl-2表达水平的相关性[J]. 中华肺部疾病杂志(电子版), 2022, 15(04): 527-529.
[5] 林红卫, 李王平, 金发光. PM2.5激活HIF-1α-NF-κB/VEGF通路对肺损伤的影响[J]. 中华肺部疾病杂志(电子版), 2022, 15(03): 316-322.
[6] 李公豪, 赵艳丽, 彭中兴, 尹德录, 赵云峰. 下调血管生成素样蛋白7表达对血管紧张素Ⅱ介导的血管平滑肌细胞炎症反应的影响[J]. 中华细胞与干细胞杂志(电子版), 2022, 12(02): 93-99.
[7] 蒋梦洁, 钱治军, 徐思, 梁伟. 低强度脉冲超声波治疗冻结肩模型兔的实验研究[J]. 中华肩肘外科电子杂志, 2023, 11(01): 30-34.
[8] 刘一, 文旖旎, 吴映辉. 过敏性紫癜患儿外周血辅助性T细胞、调节性T细胞细胞因子与肾损害的相关性分析[J]. 中华肾病研究电子杂志, 2023, 12(05): 271-275.
[9] 吴震宇, 胡亚芬, 董晓芬, 马远方. 血清CTGF、TGF-β1、MMP2水平对糖尿病肾病肾间质纤维化的预测分析[J]. 中华肾病研究电子杂志, 2022, 11(06): 332-337.
[10] 崔宏宇, 杨一佺, 郭黎霞, 吕爱国, 张志宏, 张新, 杨艳萍, 申然, 连丽英, 曹志刚, 王立芳, 胡建华, 范肃洁. 改良Ahmed青光眼引流阀植入术治疗闭角期新生血管性青光眼疗效的临床研究[J]. 中华眼科医学杂志(电子版), 2023, 13(02): 76-81.
[11] 吴晓翔, 杨波, 李景漩, 张凤玲, 郭桂辉, 郑少培. 脐动脉超声检查联合NLR、sFlt-1/PLGF对妊娠高血压综合征患者不良妊娠结局的预测价值[J]. 中华临床医师杂志(电子版), 2023, 17(03): 266-271.
[12] 贺芳. 易栓症和子痫前期[J]. 中华产科急救电子杂志, 2023, 12(01): 6-11.
[13] 武建利, 张丽姿, 李玲, 单婷, 王晓燕, 卢瑛, 许君君, 沈霞, 佐合热古丽·赛麦提, 石纪红, 朱启英, 杜丽丽. 新疆不同民族妊娠期高血压疾病患者1162例临床分析[J]. 中华产科急救电子杂志, 2022, 11(03): 152-157.
[14] 颜凡辉, 赵明俐, 李颖, 郭方明, 詹景冬, 赵英杰, 王阳, 张艳芬, 赵笑梅. 急性冠脉综合征患者冠脉血管病变程度与血清TNF-α、VEGF水平相关性研究[J]. 中华诊断学电子杂志, 2023, 11(03): 158-164.
[15] 何圣清, 袁唯唯, 孟莞瑞, 符青松, 郑晓斌, 武红梅. 达格列净联合二甲双胍治疗对早期2型糖尿病肾病患者肾小管功能和血清Klotho的影响[J]. 中华肥胖与代谢病电子杂志, 2022, 08(04): 236-242.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号