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中华妇幼临床医学杂志(电子版) ›› 2013, Vol. 09 ›› Issue (05) : 636 -641. doi: 10.3877/cma.j.issn.1673-5250.2013.05.015

所属专题： 文献；

论著

金纳多、姜黄素对缺氧缺血性脑损伤新生大鼠脑基质金属蛋白酶-9表达的影响
林迳苍1, 黄煌1,*(), 张少明2, 许相洋1, 黄建强3,*()   
  1. 1. 362000　福建泉州，福建泉州医学高等专科学校
    2. 泉州皮防医院
    3. 福建医科大学附二医院
  • 收稿日期:2013-04-28 修回日期:2013-09-01 出版日期:2013-10-01
  • 通信作者: 黄煌, 黄建强

Effects of Ginaton and Curcumin on Matrix Metalloproteinase-9 Expression Changes of Newborn Rats With Hypoxic-Ischemic Encephalopathy

Jing-cang LIN1, Huang HUANG1(), Shao-ming ZHANG2, Xiang-yang XU1, Jian-qiang HUANG3()   

  1. 1. Quanzhou Medical College, Quanzhou 362000, Fujian Province, China
  • Received:2013-04-28 Revised:2013-09-01 Published:2013-10-01
  • Corresponding author: Huang HUANG, Jian-qiang HUANG
  • About author:
    (Corresponding author: HUNAG Huang, Email: )
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林迳苍, 黄煌, 张少明, 许相洋, 黄建强. 金纳多、姜黄素对缺氧缺血性脑损伤新生大鼠脑基质金属蛋白酶-9表达的影响[J]. 中华妇幼临床医学杂志(电子版), 2013, 09(05): 636-641.

Jing-cang LIN, Huang HUANG, Shao-ming ZHANG, Xiang-yang XU, Jian-qiang HUANG. Effects of Ginaton and Curcumin on Matrix Metalloproteinase-9 Expression Changes of Newborn Rats With Hypoxic-Ischemic Encephalopathy[J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2013, 09(05): 636-641.

目的

探讨新生SD大鼠缺氧缺血性脑损伤(HIE)后，应用金纳多及姜黄素治疗后其脑组织中基质金属蛋白酶(MMP)-9的表达变化趋势。

方法

将新生7 d SD大鼠90只随机分为5组，假手术组、HIE组、HIE＋金纳多干预组(金纳多治疗组)、HIE＋姜黄素干预组(姜黄素治疗组)及HIE＋金纳多＋姜黄素干预组，每组各18只。HIE模型制备成功后处死各动物，观察HIE后6 h，24 h和48 h，观察脑组织MMP的表达。

结果

HIE模型制备成功后，各组SD大鼠行为出现不同改变。金纳多治疗组、姜黄素治疗组和综合治疗组于各时间亚组MMP-9免疫阳性细胞平均灰度值分别与假手术组、HIE组比较，差异均有统计学意义(P<0.01)；金纳多治疗组、姜黄素治疗组各时间亚组分别与综合治疗组比较，差异亦有统计学意义(P<0.01)。

结论

金纳多＋姜黄素对新生SD大鼠HIE后神经系统功能恢复具有更为明显的改善作用。

关键词: 金纳多, 姜黄素, 缺氧缺血性脑损伤, 新生大鼠, 基质金属蛋白酶-9
Objective

To explore the matrix metalloproteinase (MMP)-9 expression changes of newborn rats with hypoxic-ischemic encephalopathy (HIE) after ginaton and curcumin treatment.

Methods

Ninety newborn 7 days Sprague-Dawley rats were randomly divided into five groups, sham operation control group (n=18), HIE group(n=18), HIE+ ginaton intervention group (ginaton group, n=18), HIE+ curcumin intervention group(curcumin group, n=18), HIE+ ginaton+ curcumin intervention group(comprehensive group, n=18). Those five groups were further divided into three sub-group after HIE, 6 h sub-group, 24 h sub-group, 48 h sub-group. The change tendency of MMP-9 after treating by ginaton and curcumin were observed.

Results

Compared with sham group and HIE group, the 6 h, 24 h, and 48 h sub-group of immunoreactive cells in the average gray value among ginaton group, curcumin group comprehensive group had statistically significant difference (P<0.01), and there still had statistically significant difference between ginaton group and comprehensive group, curcumin group and comprehensive group (P<0.01).

Conclusion

The effects of ginaton combined with curcumin had obvious improvement on the nervous system functions recovery after HIE.

Key words: ginaton, curcumin, hypoxic-ischemic encephalopathy, newborn rats, matrix metalloproteinase-9
表1 各组MMP-9免疫阳性细胞平均灰度值比较(±s)
Table 1 The expression of MMP-9 protein in each group(±s)
组别 n 6 h 24 h 48 h
HIE组 6 150.64±3.92 155.98±2.37 152.90±2.52
金纳多治疗组 6 122.30±4.71ab 128.26±4.65ab 125.27±2.91ab
姜黄素治疗组 6 123.27±3.91ab 128.99±3.85ab 127.33±4.85ab
综合治疗组 6 111.27±3.76abcd 119.56±5.44abcd 117.82±2.55abcd
假手术组 6 107.26±3.51 95.48±2.72 109.47±4.23
t ? 13.10 22.21 14.55
P ? 0.001 0.001 0.001
图1 五组大鼠不同亚组脑组织MMP-9阳性细胞平均灰度值折线图
Figure 1 Five groups of brain tissue of rats with different subgroups MMP - 9 positive cell average grey value line chart
图4 金纳多治疗组HIE后6 h MMP-9的表达 (DAB×400)
Figure 4 Skinner than 6 h after treatment group HIE the expression of MMP-9 (DAB×400)
图7 假手术组HIE后24 h MMP-9的表达 (DAB×400)
Figure 7 Control HIE 24 h after the expression of MMP-9 (DAB×400)
图10 姜黄素治疗组HIE后24 h MMP-9的表达 (DAB×400)
Figure 10 24 h after curcumin treatment group HIE the expression of MMP-9 (DAB×400)
图13 HIE组HIE后48 h MMP-9的表达 (DAB×400)
Figure 13 HIE group 48 h after the expression of MMP-9 (DAB×400)
图16 综合治疗组HIE后48 h MMP-9的表达 (DAB×400)
Figure 16 Comprehensive treatment group 48 h after HIE the expression of MMP-9 (DAB×400)
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