探讨华蟾素(cinobufagin)对体外培养子宫内膜癌HHUA细胞芳香化酶和雌二醇(estradiol，E₂)表达的影响和意义。

30例原代培养的正常子宫内膜组织和30例子宫内膜癌HHUA细胞分别取自2007年至2008年因功能性子宫出血(功血)而在本院诊刮和因子宫内膜癌在本院手术治疗患者。其组织学检查分别确诊为增生期内膜和子宫内膜癌HHUA细胞，体外(in vitro)培养正常子宫内膜细胞(A组，n＝30)和子宫内膜癌HHUA细胞。再将30例子宫内膜癌患者的子宫内膜癌HHUA细胞分成两部分，一部分纳入子宫内膜癌HHUA细胞组(B组，n＝30)，一部分用华蟾素干预后，纳入子宫内膜癌HHUA细胞华蟾素干预组(C组，n＝30)，应用RT–PCR检测芳香化酶表达，发光免疫法检测雌二醇表达，流式细胞仪检测细胞凋亡。

正常子宫内膜细胞(A组)不表达芳香化酶mRNA(aromatase mRNA)，不合成雌二醇，细胞凋亡率为(8.02±0.93)%；子宫内膜癌HHUA细胞(B组)表达芳香化酶mRNA，可合成雌二醇，细胞凋亡率为(1.08±0.21)%，低于正常子宫内膜细胞 (P<0.05)。华蟾素干预(C组)后，子宫内膜癌HHUA细胞芳香化酶mRNA的表达和雌二醇的合成均下降，细胞凋亡率为(27.14±4.12)%，高于B组(P<0.05)。

子宫内膜癌HHUA细胞可利用芳香化酶合成内源性雌二醇，华蟾素可抑制子宫内膜癌HHUA细胞芳香化酶mRNA的表达和雌二醇的合成，抑制癌细胞增殖。

To investigate the effects and significance of cinobufagin on the expression of aromatase and estrodiol(E₂) in the endometrial carcinoma HHUA cells cultured in vitro.

The primary culture of normal endometrial cells were taken from patients who did diagnostic curettage for functional uterine bleeding(FUB) in our hospital from 2007 to 2008, and the pathology revealed proliferative endometrial cells as the normal endometrial cells(group A, n=30). The pathology revealed as the endometrial carcinoma HHUA cells which were divided into two parts. Half of them were classified into endometrial carcinoma HHUA cells group (group B, n=30), and half were cultured in vitro, after the action of cinobufagin into endometrial carcinoma HHUA cells action by cinobufagin group (group C, n=30). The expression of aromatase was measured by RT-PCR, the expression of estrodiol was determined by immunofluorometry, and the cellular apoptosis was tested by flow cytometry.

Group A neither expressed aromatase mRNA nor synthesize estrodiol, the cellular apoptotic rate was (8.02±0.93)%. Group B could both express aromatase mRNA and synthesize estradiol by the aromatase, and the cellular apoptotic rate was (1.08±0.21)%, which was lower than that of group A(P<0.05). After the action of cinobufagin(group C), aromatase mRNA expression and estrodiol synthesis of endometrial carcinoma HHUA cells were significantly down-regulated, the cellular apoptotic rate was (27.14±4.12)%, which was lower than that of group B (P<0.05).

The endometrial carcinoma HHUA cells can synthesize endogenous estradiol by aromatase. The cinobufagin may inhibit the expression of aromatase mRNA and the synthesis of the estrodiol, resulting the inhibition of endometrial carcinoma cells' proliferation.