观察新型血管紧张素转化酶抑制剂(angiotension－converting enzyme inhibitor，ACEI)——福辛普利(fosinopril，FOS)对脂多糖(lipopolysaccharide，LPS)诱导的大鼠肾小球系膜细胞(glomerular mesangial cell, GMC)增殖及转化生长因子(transforming growth factor, TGF)β₁表达的影响。

建立体外培养大鼠肾小球系膜细胞，鉴定后3～10代用于实验。实验分为5组：对照组(Ctrl组)、脂多糖刺激组(LPS组)、福辛普利高、中、低剂量组(分别为FOS 1组、FOS 2组和FOS 3组)，分别于24 h和48 h两个时间点甲基噻唑基四唑(methyl thiazolyl tetrazolium，MTT)比色法测定各组细胞增殖情况；于6 h，12 h，24 h后酶联免疫测定(enzyme－linked immunosorbent－assay, ELISA)法测定细胞培养上清液中转化生长因子β₁蛋白的表达量，荧光半定量RT－PCR法检测转化生长因子β₁ mRNA表达的变化。

脂多糖可诱导大鼠肾小球系膜细胞增殖，福辛普利可抑制脂多糖诱导的大鼠肾小球系膜细胞增殖；正常培养的大鼠肾小球系膜细胞均有一定量的转化生长因子β₁蛋白分泌和mRNA表达；LPS组在各时间点转化生长因子β₁蛋白分泌和mRNA表达均高于Ctrl组(P<0.01)，而FOS 1组、FOS 2组、FOS 3组转化生长因子β₁蛋白分泌和mRNA表达均低于LPS组(P<0.01)。

脂多糖可诱导大鼠肾小球系膜细胞增殖。福辛普利可抑制脂多糖诱导的大鼠肾小球系膜细胞增殖。福辛普利能够从蛋白水平和mRNA水平抑制脂多糖诱导的大鼠肾小球系膜细胞转化生长因子β₁的表达，为福辛普利的肾脏保护作用提供理论依据。

To observe effects of fosinopril (FOS) on proliferation and expression of transforming growth factor(TGF) β₁ of rat glomerular mesangial cell (GMC) induced by lipopolysaccharide(LPS).

After establishing the cultured glomerular mesangial cells of rat in vitro, passages 3~10 of cells were used in the experiment after identification. The experiment included 5 groups: control group(Ctrl group), lipopolysaccharide group(LPS group), high dose fosinopril group(FOS 1 group), middle dose fosinopril group(FOS 2 group) and low dose fosinopril group(FOS 3 group). Glomerular mesangial cells proliferation were detected by methyl thiazolyl tetrazolium (MTT) incorporation method at 24 h and 48 h, and the change of transforming growth factor β₁ protein secretion was detected by the enzyme-linked immunosorbent-assay (ELISA). The change of transforming growth factor β₁ mRNA expression was detected by semi-quantitative real-time(RT)-PCR.

Lipopolysaccharide can induce the glomerular mesangial cell proliferation, fosinopril can inhibit the effect of proliferation induced by lipopolysaccharide. Gomerular mesangial cells had transforming growth factor β₁ protein secretion and mRNA expression at certain level in normal culture medium, transforming growth factor β₁ protein secretion and mRNA expression were higher significantly in LPS group than those in Ctrl group (P<0.01), but lower significantly in all of fosinopril groups than those in LPS group (P<0.01).

Fosinopril can inhibit the effect of proliferation and the expression of transforming growth factor β₁ in glomerular mesangial cells at the mRNA and protein levels induced by lipopolysaccharide, and provides the theoretical evidence for the renal protection of fosinopril.