建立高效的围生期沙眼衣原体(Chlamydia trachomatis, CT)流行病学调查批处理方法——混合尿液(pooling urine)质粒缺口-连接酶链反应-酶联免疫吸附法(gap-ligase chain reaction-enzyme linked immunoabsorbent assay，gap-LCR-ELISA)。

比较质粒gap-LCR-ELISA对不同体积孕妇尿液混合库(4×，6×，8×，10×)及单份样本(1×)检出CT感染的灵敏度和特异度。

质粒gap-LCR-ELISA检测4，6，8，10人份混合尿样中的CT感染均可达到100%的特异度，灵敏度分别为95%，85%，70%和65%，各组实验成本下降的比例均超过40%。

4人份孕妇混合尿液质粒gap-LCR-ELISA的检测效能，等同于对单份标本的独立检测，为该法最适宜的标本混合策略，在大规模流行病学调查中具有应用价值。

To construct an effective pooling urine plasmid gap-LCR-ELISA method for large-scale epidemic screening of perinatal Chlamydia trachomatis (CT) infection.

The most optimal pooling size (4×, 6×, 8×, 10×) was chosen by comparing the sensitivity, specificity and cost-effectiveness with single urine detection.

Pooling urine by 4×, 6×, 8×, 10× size to gap-LCR-ELISA resulted in a satisfying specificity up to 100%, but the sensitivity declined with the increase of pooling volume (95%, 85%, 70% and 65%, respectively). And a significant cost reduction of more than40% was obtained in all the groups.

4× size pooling urine strategy could achieve the same detecting capability as that of individual specimen examined by plasmid gap-LCR-ELISA, which is quite valuable in large scale epidemic screening for CT infection.