探讨缺氧诱导因子在妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy, ICP)患者胎盘上的表达及意义。

通过体外缺氧/复氧培养胎盘绒毛组织，釆用免疫组织化学染色技术，研究ICP患者和正常晚孕妇女胎盘上缺氧诱导因子(hypoxia-inducible transcription factor，HIF)HIF-1α，HIF-2α表达的变化。

①ICP组缺氧(2% O₂)4 h与常氧(21% O₂)下比较，HIF-1α表达明显增加(P<0.05)。复氧15 min，两组HIF-1α均较缺氧4 h的表达明显降低(P<0.05)。②正常组缺氧4 h，HIF-2α的表达较常氧下明显升高(P<0.01)；复氧15 min,HIF-2α的表达较缺氧4 h明显降低(P<0.01)。而缺氧/复氧过程中，ICP组HIF-2α的变化差异无显著意义(P>0.05)。

ICP胎儿缺氧时，HIF-1α的应激性升高，对其适应缺氧有重要意义，可能是分子水平上ICP急性缺氧的敏感指标。急性缺氧时，ICP胎盘上HIF- 2α的功能障碍可能导致不良妊娠结局。

We investigated the expression of the hypoxia-inducible transcription factor(HIF)in placenta of pregnant women with intrahepatic cholestasis of pregnancy(ICP).

HIF-1α and HIF-2α Proteins from villous explant culture under hypoxia condition were probed by immuno-histochemistry methods.

① HIF-1α protein in the placental villous tissues cultured for 4 h under hypoxic condition(2% O₂)were detected in two groups.The expressions of HIF-1α increased in the placentae of pregnant women with ICP(P<0.05).With the O₂ concentration recovered, the high O₂ concentration(21% O₂)decreased the expression HIF-1α in 15 min(P<0.05)in two groups.②The expression of HIF-2α was highly increased under hypoxia condition than that under normal concentration O₂(P<0.01); 21 % O₂ decreased the expression HIF-2α in 15 min(P<0.01)in normal group.But no significant difference was revealed in the change of the HIF-2α in ICP(P > 0.05).

Our research suggested that as a sensitive index, high increase of HIF-la may play an important pole in adapting hypoxia and the abnormal change of HIF-2α may also bring bad pregnancy outcome in ICP under hypoxic conditions.