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中华妇幼临床医学杂志(电子版) ›› 2006, Vol. 02 ›› Issue (03) : 130 -133. doi: 10.3877/cma.j.issn.1673-5250.2006.03.103

论著

微胶囊化儿茶素对阿霉素诱导的肾病大鼠黄嘌呤氧化酶、羟自由基与脂质过氧化产物丙二醛的影响
何小解, 易著文, 莫双红, 党西强, 白海涛   
  1. 长沙中南大学湘雅二医院小儿肾脏病研究室,湖南省小儿肾脏病临床中心(长沙,410011)
  • 出版日期:2006-05-21
  • 基金资助:
    湖南省自然科学基金(02JJXY3020); 湖南省教育厅重点项目基金资助(02A015)

Effect of catechin microcapsulation on xanthe oxidase, hydroxy radical and malonydialdehyde in rat with nephrotic syndrome induced-adriblastine

Xiao-jie HE, Zhu-wen YI, Shuang-hong MO, Xi-qiang DANG, Hai-tao BAI   

  1. Laboratory of Pediatric Nephrology, the Institute of Pediatrics, the Second Xiangya Hospital of Central South University. Changsha, 410011, Hunan Province, China
  • Published:2006-05-21
引用本文:

何小解, 易著文, 莫双红, 党西强, 白海涛. 微胶囊化儿茶素对阿霉素诱导的肾病大鼠黄嘌呤氧化酶、羟自由基与脂质过氧化产物丙二醛的影响[J/OL]. 中华妇幼临床医学杂志(电子版), 2006, 02(03): 130-133.

Xiao-jie HE, Zhu-wen YI, Shuang-hong MO, Xi-qiang DANG, Hai-tao BAI. Effect of catechin microcapsulation on xanthe oxidase, hydroxy radical and malonydialdehyde in rat with nephrotic syndrome induced-adriblastine[J/OL]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2006, 02(03): 130-133.

目的

探讨微胶囊化儿茶素对阿霉素诱导的肾病大鼠黄嘌呤氧化酶(xanthe oxidase, XOD),羟自由基(hydroxy radical, OH)与脂质过氧化产物丙二醛(malonydialdehyde,MDA)的影响。

方法

将60只雌性SD大鼠随机分为对照组、肾病组、地塞米松组、维生素E(vitamin E)组、儿茶素组和微胶囊化儿茶素组,共计6组,尾静脉一次性注射阿霉素(5 mg/kg)制备肾病模型。实验第6周末杀鼠取尿、血、肾及肝脏,利用生化法测定血、肾及肝脏XOD,·OH与MDA含量,利用考马斯亮蓝法测定24 h尿蛋白的排泄量。

结果

微胶囊组大鼠肝脏中XOD,·OH与MDA含量均显著低于儿茶素组、地塞米松组与vitamin E组;在血清与肾脏中XOD, ·OH与MDA含量均显著低于儿茶素组、vitamin E组,高于地塞米松组。实验末24 h尿蛋白排泄量,微胶囊组显著低于儿茶素组。

结论

微胶囊化儿茶素可能是通过抑制XOD活性,有效清除·OH,抑制MDA达到降低肾病大鼠24 h尿蛋白排泄的目的。

Objective

To study the effect of catechin microcapsule on xanthe oxidase(XOD), hydroxy radical(OH)and malonydialdehyde(MDA)in rats with nephrotic syndrome induced-adriblastine.

Methods

60 female SD rats are randomly distributed in control group, nephrotic syndrome group, gloucortcoid group, vitamin E group, catechin group and catechin microcapsule group. Nephrotic syndrome rats are caused by injecting adriblastine. before rats were killed, we collect urine, blood, liver tissue and kidney tissue. XOD, ·OH and MDA concentration were detected by biochemistry assay, 24 hrs urinary protein excretion were detected by Comb's bright blueness assay.

Results

Liver tissue XOD, ·OH and MDA concentration of rats in microcapsule group were lower than that of in catechin group, gloucortcoid group and vitamin E group, kidney tissue and serum XOD, OH and MDA concentration of rats in microcapsule group were lower than that of in catechin group and vitamin E group, were higher than that of in gloucortcoid group. 24 hrs urinary protein excretion of rats in microcapsule group were lower than that of in catechin group in the end of experiment.

Conclusion

Catechin microcapsule could succeedly reduce 24 hrs urinary protein excretion in rats with nephrotic syndrome induced-adriblastine might be achieved through restraining XOD, eliminating OH efficiency, restraining MDA.

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